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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Escherichia coli O17:K52:H18 Anaerobic nitric oxide reductase transcription regulator norR (norR) CSB-YP483665EOG
CSB-EP483665EOG
CSB-BP483665EOG
CSB-MP483665EOG
CSB-EP483665EOG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O17:K52:H18 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF) CSB-YP483666EOG
CSB-EP483666EOG
CSB-BP483666EOG
CSB-MP483666EOG
CSB-EP483666EOG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O17:K52:H18 Enolase (eno) CSB-YP483667EOG
CSB-EP483667EOG
CSB-BP483667EOG
CSB-MP483667EOG
CSB-EP483667EOG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O17:K52:H18 Ribosomal RNA large subunit methyltransferase M (rlmM) CSB-YP483668EOG
CSB-EP483668EOG
CSB-BP483668EOG
CSB-MP483668EOG
CSB-EP483668EOG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O17:K52:H18 D-phenylhydantoinase (hyuA) CSB-YP483669EOG
CSB-EP483669EOG
CSB-BP483669EOG
CSB-MP483669EOG
CSB-EP483669EOG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O17:K52:H18 UPF0149 protein ygfB (ygfB) CSB-YP483670EOG
CSB-EP483670EOG
CSB-BP483670EOG
CSB-MP483670EOG
CSB-EP483670EOG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O17:K52:H18 UPF0246 protein yaaA (yaaA) CSB-YP483671EOG
CSB-EP483671EOG
CSB-BP483671EOG
CSB-MP483671EOG
CSB-EP483671EOG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O17:K52:H18 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH) CSB-YP483672EOG
CSB-EP483672EOG
CSB-BP483672EOG
CSB-MP483672EOG
CSB-EP483672EOG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O17:K52:H18 Crotonobetainyl-CoA dehydrogenase (caiA) CSB-YP483673EOG
CSB-EP483673EOG
CSB-BP483673EOG
CSB-MP483673EOG
CSB-EP483673EOG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O17:K52:H18 Protein ApaG (apaG) CSB-YP483674EOG
CSB-EP483674EOG
CSB-BP483674EOG
CSB-MP483674EOG
CSB-EP483674EOG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O17:K52:H18 Ribulokinase (araB) CSB-YP483675EOG
CSB-EP483675EOG
CSB-BP483675EOG
CSB-MP483675EOG
CSB-EP483675EOG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O17:K52:H18 UDP-N-acetylmuramate--L-alanine ligase (murC) CSB-YP483676EOG
CSB-EP483676EOG
CSB-BP483676EOG
CSB-MP483676EOG
CSB-EP483676EOG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O17:K52:H18 Iron--sulfur cluster insertion protein ErpA (erpA) CSB-YP483677EOG
CSB-EP483677EOG
CSB-BP483677EOG
CSB-MP483677EOG
CSB-EP483677EOG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O17:K52:H18 30S ribosomal protein S2 (rpsB) CSB-YP483678EOG
CSB-EP483678EOG
CSB-BP483678EOG
CSB-MP483678EOG
CSB-EP483678EOG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O17:K52:H18 Ribonuclease HII (rnhB) CSB-YP483679EOG
CSB-EP483679EOG
CSB-BP483679EOG
CSB-MP483679EOG
CSB-EP483679EOG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O17:K52:H18 Phosphoheptose isomerase (gmhA) CSB-YP483680EOG
CSB-EP483680EOG
CSB-BP483680EOG
CSB-MP483680EOG
CSB-EP483680EOG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O17:K52:H18 50S ribosomal protein L31 type B (rpmE2) CSB-YP483681EOG
CSB-EP483681EOG
CSB-BP483681EOG
CSB-MP483681EOG
CSB-EP483681EOG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O17:K52:H18 2,3-dihydroxyphenylpropionate/2,3-dihydroxicinnamic acid 1,2-dioxygenase (mhpB) CSB-YP483682EOG
CSB-EP483682EOG
CSB-BP483682EOG
CSB-MP483682EOG
CSB-EP483682EOG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O17:K52:H18 Shikimate kinase 2 (aroL) CSB-YP483683EOG
CSB-EP483683EOG
CSB-BP483683EOG
CSB-MP483683EOG
CSB-EP483683EOG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O17:K52:H18 6,7-dimethyl-8-ribityllumazine synthase (ribH) CSB-YP483684EOG
CSB-EP483684EOG
CSB-BP483684EOG
CSB-MP483684EOG
CSB-EP483684EOG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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