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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Escherichia fergusonii UPF0208 membrane protein YfbV (yfbV), partial CSB-YP485026EOR1
CSB-EP485026EOR1
CSB-BP485026EOR1
CSB-MP485026EOR1
CSB-EP485026EOR1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase (menD), partial CSB-YP485027EOR
CSB-EP485027EOR
CSB-BP485027EOR
CSB-MP485027EOR
CSB-EP485027EOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii Probable 4-deoxy-4-formamido-L-arabinose-phosphoundecaprenol deformylase ArnD (arnD) CSB-YP485028EOR
CSB-EP485028EOR
CSB-BP485028EOR
CSB-MP485028EOR
CSB-EP485028EOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii UDP-4-amino-4-deoxy-L-arabinose--oxoglutarate aminotransferase (arnB) CSB-YP485029EOR
CSB-EP485029EOR
CSB-BP485029EOR
CSB-MP485029EOR
CSB-EP485029EOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP485030EOR
CSB-EP485030EOR
CSB-BP485030EOR
CSB-MP485030EOR
CSB-EP485030EOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii 1-deoxy-D-xylulose-5-phosphate synthase (dxs), partial CSB-YP485031EOR
CSB-EP485031EOR
CSB-BP485031EOR
CSB-MP485031EOR
CSB-EP485031EOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii Acyl carrier protein phosphodiesterase (acpH) CSB-YP485032EOR
CSB-EP485032EOR
CSB-BP485032EOR
CSB-MP485032EOR
CSB-EP485032EOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii Peptide methionine sulfoxide reductase MsrA (msrA) CSB-YP485033EOR
CSB-EP485033EOR
CSB-BP485033EOR
CSB-MP485033EOR
CSB-EP485033EOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii UPF0102 protein yraN (yraN) CSB-YP485034EOR
CSB-EP485034EOR
CSB-BP485034EOR
CSB-MP485034EOR
CSB-EP485034EOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii Ribosomal protein S12 methylthiotransferase RimO (rimO) CSB-YP485035EOR
CSB-EP485035EOR
CSB-BP485035EOR
CSB-MP485035EOR
CSB-EP485035EOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii Serine--tRNA ligase (serS) CSB-YP485036EOR
CSB-EP485036EOR
CSB-BP485036EOR
CSB-MP485036EOR
CSB-EP485036EOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii Chromosome partition protein MukB (mukB), partial CSB-YP485037EOR
CSB-EP485037EOR
CSB-BP485037EOR
CSB-MP485037EOR
CSB-EP485037EOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii Prolipoprotein diacylglyceryl transferase (lgt), partial CSB-YP485038EOR1
CSB-EP485038EOR1
CSB-BP485038EOR1
CSB-MP485038EOR1
CSB-EP485038EOR1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii Phosphopentomutase (deoB) CSB-YP485039EOR
CSB-EP485039EOR
CSB-BP485039EOR
CSB-MP485039EOR
CSB-EP485039EOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii Dihydroorotate dehydrogenase (quinone) CSB-YP485040EOR
CSB-EP485040EOR
CSB-BP485040EOR
CSB-MP485040EOR
CSB-EP485040EOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii Flagellar transcriptional regulator FlhD (flhD) CSB-YP485041EOR
CSB-EP485041EOR
CSB-BP485041EOR
CSB-MP485041EOR
CSB-EP485041EOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii UPF0267 protein yqfB (yqfB) CSB-YP485042EOR
CSB-EP485042EOR
CSB-BP485042EOR
CSB-MP485042EOR
CSB-EP485042EOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii Chromosome initiation inhibitor (iciA) CSB-YP485043EOR
CSB-EP485043EOR
CSB-BP485043EOR
CSB-MP485043EOR
CSB-EP485043EOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii Crossover junction endodeoxyribonuclease RuvC (ruvC) CSB-YP485044EOR
CSB-EP485044EOR
CSB-BP485044EOR
CSB-MP485044EOR
CSB-EP485044EOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii UPF0266 membrane protein yobD (yobD) CSB-YP485045EOR
CSB-EP485045EOR
CSB-BP485045EOR
CSB-MP485045EOR
CSB-EP485045EOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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