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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Macaca fascicularis D-amino-acid oxidase (DAO) CSB-YP006494MOV
CSB-EP006494MOV
CSB-BP006494MOV
CSB-MP006494MOV
CSB-EP006494MOV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus sanguinis Adenine phosphoribosyltransferase (apt) CSB-YP001954SUR
CSB-EP001954SUR
CSB-BP001954SUR
CSB-MP001954SUR
CSB-EP001954SUR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus sanguinis CTP synthase (pyrG), partial CSB-YP006176SUR
CSB-EP006176SUR
CSB-BP006176SUR
CSB-MP006176SUR
CSB-EP006176SUR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus sanguinis Argininosuccinate lyase (argH) CSB-YP002213SUR
CSB-EP002213SUR
CSB-BP002213SUR
CSB-MP002213SUR
CSB-EP002213SUR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanoculleus marisnigri Argininosuccinate lyase (argH) CSB-YP002213MNU
CSB-EP002213MNU
CSB-BP002213MNU
CSB-MP002213MNU
CSB-EP002213MNU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanoculleus marisnigri Ornithine carbamoyltransferase (argF) CSB-YP017270MNU
CSB-EP017270MNU
CSB-BP017270MNU
CSB-MP017270MNU
CSB-EP017270MNU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica GTP cyclohydrolase 1 (folE) CSB-YP009317STL
CSB-EP009317STL
CSB-BP009317STL
CSB-MP009317STL
CSB-EP009317STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Glutaminase (glsA) CSB-YP009528STL
CSB-EP009528STL
CSB-BP009528STL
CSB-MP009528STL
CSB-EP009528STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Argininosuccinate lyase (argH) CSB-YP002213STL
CSB-EP002213STL
CSB-BP002213STL
CSB-MP002213STL
CSB-EP002213STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum CTP synthase (pyrG), partial CSB-YP006176DUT
CSB-EP006176DUT
CSB-BP006176DUT
CSB-MP006176DUT
CSB-EP006176DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum GTP cyclohydrolase 1 (folE) CSB-YP009317DUT
CSB-EP009317DUT
CSB-BP009317DUT
CSB-MP009317DUT
CSB-EP009317DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mustela putorius furo Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) CSB-YP009232MOH
CSB-EP009232MOH
CSB-BP009232MOH
CSB-MP009232MOH
CSB-EP009232MOH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Transmembrane protein 2 (tmem2), partial CSB-YP023791DIL
CSB-EP023791DIL
CSB-BP023791DIL
CSB-MP023791DIL
CSB-EP023791DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Scheffersomyces stipitis Phosphatidylethanolamine N-methyltransferase (CHO2), partial CSB-YP017780SXM
CSB-EP017780SXM
CSB-BP017780SXM
CSB-MP017780SXM
CSB-EP017780SXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii CTP synthase (pyrG), partial CSB-YP006176AUD
CSB-EP006176AUD
CSB-BP006176AUD
CSB-MP006176AUD
CSB-EP006176AUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii Uroporphyrinogen decarboxylase (hemE) CSB-YP025678AUD
CSB-EP025678AUD
CSB-BP025678AUD
CSB-MP025678AUD
CSB-EP025678AUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei CTP synthase (pyrG), partial CSB-YP006176BPQ
CSB-EP006176BPQ
CSB-BP006176BPQ
CSB-MP006176BPQ
CSB-EP006176BPQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Aminomethyltransferase (gcvT) CSB-YP001684BPQ
CSB-EP001684BPQ
CSB-BP001684BPQ
CSB-MP001684BPQ
CSB-EP001684BPQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum calidifontis Ornithine carbamoyltransferase (argF) CSB-YP017270PZV
CSB-EP017270PZV
CSB-BP017270PZV
CSB-MP017270PZV
CSB-EP017270PZV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 5b Adenine phosphoribosyltransferase (apt) CSB-YP001954AUF
CSB-EP001954AUF
CSB-BP001954AUF
CSB-MP001954AUF
CSB-EP001954AUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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