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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Debaryomyces hansenii Protein DSE1 (DSE1), partial CSB-YP721566DIS
CSB-EP721566DIS
CSB-BP721566DIS
CSB-MP721566DIS
CSB-EP721566DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii Endoplasmic reticulum vesicle protein 25 (ERV25), partial CSB-YP721567DIS
CSB-EP721567DIS
CSB-BP721567DIS
CSB-MP721567DIS
CSB-EP721567DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii GPI-anchored wall transfer protein 1 (GWT1), partial CSB-YP721568DIS
CSB-EP721568DIS
CSB-BP721568DIS
CSB-MP721568DIS
CSB-EP721568DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii Ubiquitin-like protein ATG12 (ATG12) CSB-YP721569DIS
CSB-EP721569DIS
CSB-BP721569DIS
CSB-MP721569DIS
CSB-EP721569DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii Pre-mRNA-splicing factor PRP46 (PRP46) CSB-YP721570DIS
CSB-EP721570DIS
CSB-BP721570DIS
CSB-MP721570DIS
CSB-EP721570DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii Altered inheritance of mitochondria protein 23, mitochondrial (AIM23) CSB-YP721571DIS
CSB-EP721571DIS
CSB-BP721571DIS
CSB-MP721571DIS
CSB-EP721571DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii Pre-rRNA-processing protein RIX1 (RIX1), partial CSB-YP721572DIS
CSB-EP721572DIS
CSB-BP721572DIS
CSB-MP721572DIS
CSB-EP721572DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii NADH-cytochrome b5 reductase 1 (CBR1), partial CSB-YP721573DIS
CSB-EP721573DIS
CSB-BP721573DIS
CSB-MP721573DIS
CSB-EP721573DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii Serine/threonine-protein kinase TEL1 (TEL1), partial CSB-YP721574DIS
CSB-EP721574DIS
CSB-BP721574DIS
CSB-MP721574DIS
CSB-EP721574DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii Transcription elongation factor SPT6 (SPT6), partial CSB-YP721575DIS
CSB-EP721575DIS
CSB-BP721575DIS
CSB-MP721575DIS
CSB-EP721575DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii Plasma membrane proteolipid 3 (PMP3), partial CSB-YP721576DIS
CSB-EP721576DIS
CSB-BP721576DIS
CSB-MP721576DIS
CSB-EP721576DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii Dol-P-Glc:Glc (2)Man (9)GlcNAc (2)-PP-Dol alpha-1,2-glucosyltransferase (ALG10), partial CSB-YP721577DIS
CSB-EP721577DIS
CSB-BP721577DIS
CSB-MP721577DIS
CSB-EP721577DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii Altered inheritance of mitochondria protein 21 (AIM21), partial CSB-YP721578DIS
CSB-EP721578DIS
CSB-BP721578DIS
CSB-MP721578DIS
CSB-EP721578DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii Peptidyl-prolyl isomerase CWC27 (CWC27) CSB-YP721579DIS
CSB-EP721579DIS
CSB-BP721579DIS
CSB-MP721579DIS
CSB-EP721579DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii Cytosolic Fe-S cluster assembly factor CFD1 (CFD1) CSB-YP721580DIS
CSB-EP721580DIS
CSB-BP721580DIS
CSB-MP721580DIS
CSB-EP721580DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii SWR1-complex protein 5 (SWC5) CSB-YP721581DIS
CSB-EP721581DIS
CSB-BP721581DIS
CSB-MP721581DIS
CSB-EP721581DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii U1 small nuclear ribonucleoprotein component SNU71 (SNU71), partial CSB-YP721582DIS
CSB-EP721582DIS
CSB-BP721582DIS
CSB-MP721582DIS
CSB-EP721582DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii Mediator of RNA polymerase II transcription subunit 20 (SRB2) CSB-YP721583DIS
CSB-EP721583DIS
CSB-BP721583DIS
CSB-MP721583DIS
CSB-EP721583DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii Mediator of RNA polymerase II transcription subunit 18 (SRB5) CSB-YP721584DIS
CSB-EP721584DIS
CSB-BP721584DIS
CSB-MP721584DIS
CSB-EP721584DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii ATPase synthesis protein 25, mitochondrial (ATP25), partial CSB-YP721585DIS
CSB-EP721585DIS
CSB-BP721585DIS
CSB-MP721585DIS
CSB-EP721585DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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