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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Propionibacterium acnes Ribonuclease HII (rnhB) CSB-YP724390PSG
CSB-EP724390PSG
CSB-BP724390PSG
CSB-MP724390PSG
CSB-EP724390PSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Propionibacterium acnes Biotin synthase (bioB) CSB-YP724391PSG
CSB-EP724391PSG
CSB-BP724391PSG
CSB-MP724391PSG
CSB-EP724391PSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Propionibacterium acnes Acetylglutamate kinase (argB) CSB-YP724392PSG
CSB-EP724392PSG
CSB-BP724392PSG
CSB-MP724392PSG
CSB-EP724392PSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Propionibacterium acnes ATP synthase subunit alpha (atpA), partial CSB-YP724393PSG
CSB-EP724393PSG
CSB-BP724393PSG
CSB-MP724393PSG
CSB-EP724393PSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Propionibacterium acnes DNA-directed RNA polymerase subunit omega (rpoZ) CSB-YP724394PSG
CSB-EP724394PSG
CSB-BP724394PSG
CSB-MP724394PSG
CSB-EP724394PSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Propionibacterium acnes Alanine--tRNA ligase (alaS), partial CSB-YP724395PSG
CSB-EP724395PSG
CSB-BP724395PSG
CSB-MP724395PSG
CSB-EP724395PSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Propionibacterium acnes 1- (5-phosphoribosyl)-5-[ (5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase (hisA) CSB-YP724396PSG
CSB-EP724396PSG
CSB-BP724396PSG
CSB-MP724396PSG
CSB-EP724396PSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Propionibacterium acnes Glutamyl-tRNA (Gln) amidotransferase subunit A (gatA), partial CSB-YP724397PSG
CSB-EP724397PSG
CSB-BP724397PSG
CSB-MP724397PSG
CSB-EP724397PSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Propionibacterium acnes tRNA dimethylallyltransferase (miaA) CSB-YP724398PSG
CSB-EP724398PSG
CSB-BP724398PSG
CSB-MP724398PSG
CSB-EP724398PSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Propionibacterium acnes Aspartate carbamoyltransferase (pyrB) CSB-YP724399PSG
CSB-EP724399PSG
CSB-BP724399PSG
CSB-MP724399PSG
CSB-EP724399PSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Propionibacterium acnes Heat-inducible transcription repressor HrcA (hrcA) CSB-YP724400PSG
CSB-EP724400PSG
CSB-BP724400PSG
CSB-MP724400PSG
CSB-EP724400PSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Propionibacterium acnes Gamma-glutamyl phosphate reductase (proA) CSB-YP724401PSG
CSB-EP724401PSG
CSB-BP724401PSG
CSB-MP724401PSG
CSB-EP724401PSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Propionibacterium acnes UvrABC system protein B (uvrB), partial CSB-YP724402PSG
CSB-EP724402PSG
CSB-BP724402PSG
CSB-MP724402PSG
CSB-EP724402PSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Propionibacterium acnes Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY), partial CSB-YP724403PSG
CSB-EP724403PSG
CSB-BP724403PSG
CSB-MP724403PSG
CSB-EP724403PSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Propionibacterium acnes Glutamate-ammonia-ligase adenylyltransferase (glnE), partial CSB-YP724404PSG
CSB-EP724404PSG
CSB-BP724404PSG
CSB-MP724404PSG
CSB-EP724404PSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Propionibacterium acnes Enolase (eno) CSB-YP724405PSG
CSB-EP724405PSG
CSB-BP724405PSG
CSB-MP724405PSG
CSB-EP724405PSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Propionibacterium acnes Glucosamine-6-phosphate deaminase (nagB) CSB-YP724406PSG
CSB-EP724406PSG
CSB-BP724406PSG
CSB-MP724406PSG
CSB-EP724406PSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Propionibacterium acnes UPF0678 fatty acid-binding protein-like protein PPA0347 (PPA0347) CSB-YP724407PSG
CSB-EP724407PSG
CSB-BP724407PSG
CSB-MP724407PSG
CSB-EP724407PSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Propionibacterium acnes tRNA (Ile)-lysidine synthase (tilS) CSB-YP724408PSG
CSB-EP724408PSG
CSB-BP724408PSG
CSB-MP724408PSG
CSB-EP724408PSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Propionibacterium acnes DNA replication and repair protein recF (recF) CSB-YP724409PSG
CSB-EP724409PSG
CSB-BP724409PSG
CSB-MP724409PSG
CSB-EP724409PSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細(xì)胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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