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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Neosartorya fischeri Mediator of RNA polymerase II transcription subunit 21 (srb7) CSB-YP013654NEZ
CSB-EP013654NEZ
CSB-BP013654NEZ
CSB-MP013654NEZ
CSB-EP013654NEZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neosartorya fischeri NADH-cytochrome b5 reductase 1 (cbr1), partial CSB-YP006318NEZ1
CSB-EP006318NEZ1
CSB-BP006318NEZ1
CSB-MP006318NEZ1
CSB-EP006318NEZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neosartorya fischeri Eukaryotic translation initiation factor 3 subunit D (NFIA_089900), partial CSB-YP007533NEZ
CSB-EP007533NEZ
CSB-BP007533NEZ
CSB-MP007533NEZ
CSB-EP007533NEZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neosartorya fischeri Mediator of RNA polymerase II transcription subunit 18 (srb5) CSB-YP013651NEZ
CSB-EP013651NEZ
CSB-BP013651NEZ
CSB-MP013651NEZ
CSB-EP013651NEZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neosartorya fischeri Mediator of RNA polymerase II transcription subunit 7 (med7) CSB-YP013667NEZ
CSB-EP013667NEZ
CSB-BP013667NEZ
CSB-MP013667NEZ
CSB-EP013667NEZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B L-threonine 3-dehydrogenase (tdh) CSB-YP023350YAK
CSB-EP023350YAK
CSB-BP023350YAK
CSB-MP023350YAK
CSB-EP023350YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B Triosephosphate isomerase (tpiA) CSB-YP024102YAK
CSB-EP024102YAK
CSB-BP024102YAK
CSB-MP024102YAK
CSB-EP024102YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B 3-ketoacyl-CoA thiolase (fadA), partial CSB-YP001117YAK
CSB-EP001117YAK
CSB-BP001117YAK
CSB-MP001117YAK
CSB-EP001117YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B Malate dehydrogenase (mdh) CSB-YP013623YAK
CSB-EP013623YAK
CSB-BP013623YAK
CSB-MP013623YAK
CSB-EP013623YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B GTPase Era (era) CSB-YP007759YAK
CSB-EP007759YAK
CSB-BP007759YAK
CSB-MP007759YAK
CSB-EP007759YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B Protoheme IX farnesyltransferase (cyoE), partial CSB-YP005824YAK
CSB-EP005824YAK
CSB-BP005824YAK
CSB-MP005824YAK
CSB-EP005824YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B Ribosome-recycling factor (frr) CSB-YP014941YAK
CSB-EP014941YAK
CSB-BP014941YAK
CSB-MP014941YAK
CSB-EP014941YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B Methionyl-tRNA formyltransferase (fmt) CSB-YP015150YAK
CSB-EP015150YAK
CSB-BP015150YAK
CSB-MP015150YAK
CSB-EP015150YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B ATP synthase subunit beta (atpD) CSB-YP002350YAK
CSB-EP002350YAK
CSB-BP002350YAK
CSB-MP002350YAK
CSB-EP002350YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B NAD-dependent malic enzyme (maeA), partial CSB-YP013633YAK
CSB-EP013633YAK
CSB-BP013633YAK
CSB-MP013633YAK
CSB-EP013633YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azoarcus sp. ATP synthase subunit b (atpF), partial CSB-YP002358AVL1
CSB-EP002358AVL1
CSB-BP002358AVL1
CSB-MP002358AVL1
CSB-EP002358AVL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azoarcus sp. GMP synthase [glutamine-hydrolyzing] (guaA), partial CSB-YP009582AVL
CSB-EP009582AVL
CSB-BP009582AVL
CSB-MP009582AVL
CSB-EP009582AVL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azoarcus sp. Thymidylate synthase (thyA) CSB-YP025393AVL
CSB-EP025393AVL
CSB-BP025393AVL
CSB-MP025393AVL
CSB-EP025393AVL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azoarcus sp. Phosphatidylserine decarboxylase proenzyme (psd) CSB-YP018033AVL
CSB-EP018033AVL
CSB-BP018033AVL
CSB-MP018033AVL
CSB-EP018033AVL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium bovis Porphobilinogen deaminase (hemC) CSB-YP010524MOJ
CSB-EP010524MOJ
CSB-BP010524MOJ
CSB-MP010524MOJ
CSB-EP010524MOJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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