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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Debaryomyces hansenii Thioredoxin reductase (TRR1) CSB-YP738192DIS
CSB-EP738192DIS
CSB-BP738192DIS
CSB-MP738192DIS
CSB-EP738192DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii Methylthioribulose-1-phosphate dehydratase (MDE1) CSB-YP738193DIS
CSB-EP738193DIS
CSB-BP738193DIS
CSB-MP738193DIS
CSB-EP738193DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii Pre-mRNA-splicing factor SPP2 (SPP2) CSB-YP738194DIS
CSB-EP738194DIS
CSB-BP738194DIS
CSB-MP738194DIS
CSB-EP738194DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii mRNA 3'-end-processing protein RNA14 (RNA14), partial CSB-YP738195DIS
CSB-EP738195DIS
CSB-BP738195DIS
CSB-MP738195DIS
CSB-EP738195DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii Invertase (INV), partial CSB-YP738196DIS
CSB-EP738196DIS
CSB-BP738196DIS
CSB-MP738196DIS
CSB-EP738196DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii DNA damage-inducible protein 1 (DDI1) CSB-YP738197DIS
CSB-EP738197DIS
CSB-BP738197DIS
CSB-MP738197DIS
CSB-EP738197DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii Helicase SWR1 (SWR1), partial CSB-YP738198DIS
CSB-EP738198DIS
CSB-BP738198DIS
CSB-MP738198DIS
CSB-EP738198DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii Lon protease homolog, mitochondrial (PIM1), partial CSB-YP738199DIS
CSB-EP738199DIS
CSB-BP738199DIS
CSB-MP738199DIS
CSB-EP738199DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii Mitochondrial escape protein 2 (YME2), partial CSB-YP738200DIS
CSB-EP738200DIS
CSB-BP738200DIS
CSB-MP738200DIS
CSB-EP738200DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii Altered inheritance of mitochondria protein 24, mitochondrial (AIM24) CSB-YP738201DIS
CSB-EP738201DIS
CSB-BP738201DIS
CSB-MP738201DIS
CSB-EP738201DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii ATP-dependent RNA helicase DBP10 (DBP10), partial CSB-YP738202DIS
CSB-EP738202DIS
CSB-BP738202DIS
CSB-MP738202DIS
CSB-EP738202DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii Ribosome-recycling factor, mitochondrial (RRF1) CSB-YP738203DIS
CSB-EP738203DIS
CSB-BP738203DIS
CSB-MP738203DIS
CSB-EP738203DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii Putative lipase ATG15 (ATG15), partial CSB-YP738204DIS
CSB-EP738204DIS
CSB-BP738204DIS
CSB-MP738204DIS
CSB-EP738204DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii Protein EFR3 (EFR3), partial CSB-YP738205DIS
CSB-EP738205DIS
CSB-BP738205DIS
CSB-MP738205DIS
CSB-EP738205DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii Eukaryotic translation initiation factor 3 subunit B (PRT1), partial CSB-YP738206DIS
CSB-EP738206DIS
CSB-BP738206DIS
CSB-MP738206DIS
CSB-EP738206DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii Serine/threonine-protein kinase SSN3 (SSN3) CSB-YP738207DIS
CSB-EP738207DIS
CSB-BP738207DIS
CSB-MP738207DIS
CSB-EP738207DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii Triosephosphate isomerase (TPI1) CSB-YP738208DIS
CSB-EP738208DIS
CSB-BP738208DIS
CSB-MP738208DIS
CSB-EP738208DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii mRNA cap guanine-N7 methyltransferase (ABD1), partial CSB-YP738209DIS
CSB-EP738209DIS
CSB-BP738209DIS
CSB-MP738209DIS
CSB-EP738209DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii Protein SDS23 (SDS23), partial CSB-YP738210DIS
CSB-EP738210DIS
CSB-BP738210DIS
CSB-MP738210DIS
CSB-EP738210DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii Signal recognition particle subunit SRP68 (SRP68), partial CSB-YP738211DIS
CSB-EP738211DIS
CSB-BP738211DIS
CSB-MP738211DIS
CSB-EP738211DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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