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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Acinetobacter sp. tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP739394AWW
CSB-EP739394AWW
CSB-BP739394AWW
CSB-MP739394AWW
CSB-EP739394AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. Urease subunit alpha (ureC), partial CSB-YP739395AWW
CSB-EP739395AWW
CSB-BP739395AWW
CSB-MP739395AWW
CSB-EP739395AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. Probable D-serine dehydratase (dsdA) CSB-YP739396AWW
CSB-EP739396AWW
CSB-BP739396AWW
CSB-MP739396AWW
CSB-EP739396AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. Putative arginyl-tRNA--protein transferase (ate) CSB-YP739397AWW
CSB-EP739397AWW
CSB-BP739397AWW
CSB-MP739397AWW
CSB-EP739397AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. Type III pantothenate kinase (coaX) CSB-YP739398AWW
CSB-EP739398AWW
CSB-BP739398AWW
CSB-MP739398AWW
CSB-EP739398AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. NADH-quinone oxidoreductase subunit K (nuoK), partial CSB-YP739399AWW1
CSB-EP739399AWW1
CSB-BP739399AWW1
CSB-MP739399AWW1
CSB-EP739399AWW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. UPF0339 protein ACIAD0721 (ACIAD0721) CSB-YP739400AWW
CSB-EP739400AWW
CSB-BP739400AWW
CSB-MP739400AWW
CSB-EP739400AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. Histidinol dehydrogenase (hisD) CSB-YP739401AWW
CSB-EP739401AWW
CSB-BP739401AWW
CSB-MP739401AWW
CSB-EP739401AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. Tryptophan synthase beta chain (trpB) CSB-YP739402AWW
CSB-EP739402AWW
CSB-BP739402AWW
CSB-MP739402AWW
CSB-EP739402AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. Histidine--tRNA ligase (hisS) CSB-YP739403AWW
CSB-EP739403AWW
CSB-BP739403AWW
CSB-MP739403AWW
CSB-EP739403AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. PKHD-type hydroxylase ACIAD0531 (ACIAD0531) CSB-YP739404AWW
CSB-EP739404AWW
CSB-BP739404AWW
CSB-MP739404AWW
CSB-EP739404AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. tRNA pseudouridine synthase A (truA) CSB-YP739405AWW
CSB-EP739405AWW
CSB-BP739405AWW
CSB-MP739405AWW
CSB-EP739405AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. Polyribonucleotide nucleotidyltransferase (pnp), partial CSB-YP739406AWW
CSB-EP739406AWW
CSB-BP739406AWW
CSB-MP739406AWW
CSB-EP739406AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. Triosephosphate isomerase (tpiA) CSB-YP739407AWW
CSB-EP739407AWW
CSB-BP739407AWW
CSB-MP739407AWW
CSB-EP739407AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. 50S ribosomal protein L7/L12 (rplL) CSB-YP739408AWW
CSB-EP739408AWW
CSB-BP739408AWW
CSB-MP739408AWW
CSB-EP739408AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (gpmI), partial CSB-YP739409AWW
CSB-EP739409AWW
CSB-BP739409AWW
CSB-MP739409AWW
CSB-EP739409AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. ATP synthase subunit beta (atpD) CSB-YP739410AWW
CSB-EP739410AWW
CSB-BP739410AWW
CSB-MP739410AWW
CSB-EP739410AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. ATP synthase subunit c (atpE), partial CSB-YP739411AWW1
CSB-EP739411AWW1
CSB-BP739411AWW1
CSB-MP739411AWW1
CSB-EP739411AWW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. Aliphatic sulfonates import ATP-binding protein SsuB (ssuB) CSB-YP739412AWW
CSB-EP739412AWW
CSB-BP739412AWW
CSB-MP739412AWW
CSB-EP739412AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. Putative aminoacrylate hydrolase RutD (rutD) CSB-YP739413AWW
CSB-EP739413AWW
CSB-BP739413AWW
CSB-MP739413AWW
CSB-EP739413AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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