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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bacillus thuringiensis subsp. konkukian Putative sporulation transcription regulator WhiA (whiA) CSB-YP740154BAAE
CSB-EP740154BAAE
CSB-BP740154BAAE
CSB-MP740154BAAE
CSB-EP740154BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Enolase (eno) CSB-YP740155BAAE
CSB-EP740155BAAE
CSB-BP740155BAAE
CSB-MP740155BAAE
CSB-EP740155BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Lipoyl synthase (lipA) CSB-YP740156BAAE
CSB-EP740156BAAE
CSB-BP740156BAAE
CSB-MP740156BAAE
CSB-EP740156BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian 1,4-alpha-glucan branching enzyme GlgB (glgB), partial CSB-YP740157BAAE
CSB-EP740157BAAE
CSB-BP740157BAAE
CSB-MP740157BAAE
CSB-EP740157BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Glycogen synthase (glgA) CSB-YP740158BAAE
CSB-EP740158BAAE
CSB-BP740158BAAE
CSB-MP740158BAAE
CSB-EP740158BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Molybdenum cofactor biosynthesis protein C (moaC) CSB-YP740159BAAE
CSB-EP740159BAAE
CSB-BP740159BAAE
CSB-MP740159BAAE
CSB-EP740159BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Septation ring formation regulator EzrA (ezrA), partial CSB-YP740160BAAE
CSB-EP740160BAAE
CSB-BP740160BAAE
CSB-MP740160BAAE
CSB-EP740160BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Transcriptional repressor NrdR (nrdR) CSB-YP740161BAAE
CSB-EP740161BAAE
CSB-BP740161BAAE
CSB-MP740161BAAE
CSB-EP740161BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Heme-degrading monooxygenase isdG (isdG) CSB-YP740162BAAE
CSB-EP740162BAAE
CSB-BP740162BAAE
CSB-MP740162BAAE
CSB-EP740162BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Porphobilinogen deaminase (hemC) CSB-YP740163BAAE
CSB-EP740163BAAE
CSB-BP740163BAAE
CSB-MP740163BAAE
CSB-EP740163BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Septum formation protein Maf (maf) CSB-YP740164BAAE
CSB-EP740164BAAE
CSB-BP740164BAAE
CSB-MP740164BAAE
CSB-EP740164BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian GTPase obg (obg) CSB-YP740165BAAE
CSB-EP740165BAAE
CSB-BP740165BAAE
CSB-MP740165BAAE
CSB-EP740165BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Queuine tRNA-ribosyltransferase (tgt) CSB-YP740166BAAE
CSB-EP740166BAAE
CSB-BP740166BAAE
CSB-MP740166BAAE
CSB-EP740166BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Alanine--tRNA ligase (alaS), partial CSB-YP740167BAAE
CSB-EP740167BAAE
CSB-BP740167BAAE
CSB-MP740167BAAE
CSB-EP740167BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian 30S ribosomal protein S20 (rpsT) CSB-YP740168BAAE
CSB-EP740168BAAE
CSB-BP740168BAAE
CSB-MP740168BAAE
CSB-EP740168BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Chaperone protein DnaJ (dnaJ) CSB-YP740169BAAE
CSB-EP740169BAAE
CSB-BP740169BAAE
CSB-MP740169BAAE
CSB-EP740169BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Probable rRNA maturation factor (BT9727_4040) CSB-YP740170BAAE
CSB-EP740170BAAE
CSB-BP740170BAAE
CSB-MP740170BAAE
CSB-EP740170BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Transcriptional regulator MntR (mntR) CSB-YP740171BAAE
CSB-EP740171BAAE
CSB-BP740171BAAE
CSB-MP740171BAAE
CSB-EP740171BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Arginine repressor (argR2) CSB-YP740172BAAE
CSB-EP740172BAAE
CSB-BP740172BAAE
CSB-MP740172BAAE
CSB-EP740172BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian N-acetyl-gamma-glutamyl-phosphate reductase (argC) CSB-YP740173BAAE
CSB-EP740173BAAE
CSB-BP740173BAAE
CSB-MP740173BAAE
CSB-EP740173BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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