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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Ashbya gossypii Diphthamide biosynthesis protein 3 (DPH3) CSB-YP741574DOT
CSB-EP741574DOT
CSB-BP741574DOT
CSB-MP741574DOT
CSB-EP741574DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Microsomal signal peptidase subunit 1 (SPC1), partial CSB-YP741575DOT1
CSB-EP741575DOT1
CSB-BP741575DOT1
CSB-MP741575DOT1
CSB-EP741575DOT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Mediator of RNA polymerase II transcription subunit 22 (SRB6) CSB-YP741576DOT
CSB-EP741576DOT
CSB-BP741576DOT
CSB-MP741576DOT
CSB-EP741576DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii GTPase-activating protein BEM3 (BEM3), partial CSB-YP741577DOT
CSB-EP741577DOT
CSB-BP741577DOT
CSB-MP741577DOT
CSB-EP741577DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Nucleolar GTP-binding protein 1 (NOG1), partial CSB-YP741578DOT
CSB-EP741578DOT
CSB-BP741578DOT
CSB-MP741578DOT
CSB-EP741578DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii 3-isopropylmalate dehydratase (LEU1), partial CSB-YP741579DOT
CSB-EP741579DOT
CSB-BP741579DOT
CSB-MP741579DOT
CSB-EP741579DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Polyadenylate-binding protein, cytoplasmic and nuclear (PAB1), partial CSB-YP741580DOT
CSB-EP741580DOT
CSB-BP741580DOT
CSB-MP741580DOT
CSB-EP741580DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Long chronological lifespan protein 2 (LCL2) CSB-YP741581DOT
CSB-EP741581DOT
CSB-BP741581DOT
CSB-MP741581DOT
CSB-EP741581DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human immunodeficiency virus type 1 group M subtype C Protein Tat CSB-YP741582HDAH
CSB-EP741582HDAH
CSB-BP741582HDAH
CSB-MP741582HDAH
CSB-EP741582HDAH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Structure-specific endonuclease subunit SLX4 (SLX4) CSB-YP741583DOT
CSB-EP741583DOT
CSB-BP741583DOT
CSB-MP741583DOT
CSB-EP741583DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Histone acetyltransferase type B catalytic subunit (HAT1) CSB-YP741584DOT
CSB-EP741584DOT
CSB-BP741584DOT
CSB-MP741584DOT
CSB-EP741584DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii AP-3 complex subunit mu (APM3) CSB-YP741585DOT
CSB-EP741585DOT
CSB-BP741585DOT
CSB-MP741585DOT
CSB-EP741585DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii RuvB-like helicase 1 (RVB1) CSB-YP741586DOT
CSB-EP741586DOT
CSB-BP741586DOT
CSB-MP741586DOT
CSB-EP741586DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii tRNA (guanine-N (7)-)-methyltransferase subunit TRM82 (TRM82) CSB-YP741587DOT
CSB-EP741587DOT
CSB-BP741587DOT
CSB-MP741587DOT
CSB-EP741587DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii UDP-N-acetylglucosamine transferase subunit ALG14 (ALG14), partial CSB-YP741588DOT
CSB-EP741588DOT
CSB-BP741588DOT
CSB-MP741588DOT
CSB-EP741588DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Diphosphomevalonate decarboxylase (MVD1) CSB-YP741589DOT
CSB-EP741589DOT
CSB-BP741589DOT
CSB-MP741589DOT
CSB-EP741589DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Ornithine decarboxylase antizyme (OAZ1) CSB-YP741590DOT
CSB-EP741590DOT
CSB-BP741590DOT
CSB-MP741590DOT
CSB-EP741590DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Bud site selection protein 4 homolog (BUD4), partial CSB-YP741591DOT
CSB-EP741591DOT
CSB-BP741591DOT
CSB-MP741591DOT
CSB-EP741591DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Protein PXR1 (PXR1) CSB-YP741592DOT
CSB-EP741592DOT
CSB-BP741592DOT
CSB-MP741592DOT
CSB-EP741592DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Mitochondrial inner membrane organizing system protein AFR743W (AFR743W), partial CSB-YP741593DOT1
CSB-EP741593DOT1
CSB-BP741593DOT1
CSB-MP741593DOT1
CSB-EP741593DOT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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