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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Vibrio vulnificus Na (+)-translocating NADH-quinone reductase subunit D (nqrD), partial CSB-YP742075VCQ1
CSB-EP742075VCQ1
CSB-BP742075VCQ1
CSB-MP742075VCQ1
CSB-EP742075VCQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus UPF0178 protein VV2566 (VV2566) CSB-YP742076VCQ
CSB-EP742076VCQ
CSB-BP742076VCQ
CSB-MP742076VCQ
CSB-EP742076VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus 30S ribosomal protein S2 (rpsB) CSB-YP742077VCQ
CSB-EP742077VCQ
CSB-BP742077VCQ
CSB-MP742077VCQ
CSB-EP742077VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus tRNA (Ile)-lysidine synthase (tilS) CSB-YP742078VCQ
CSB-EP742078VCQ
CSB-BP742078VCQ
CSB-MP742078VCQ
CSB-EP742078VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus 3-ketoacyl-CoA thiolase (fadI) CSB-YP742079VCQ
CSB-EP742079VCQ
CSB-BP742079VCQ
CSB-MP742079VCQ
CSB-EP742079VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Lipid A export ATP-binding/permease protein MsbA (msbA), partial CSB-YP742080VCQ1
CSB-EP742080VCQ1
CSB-BP742080VCQ1
CSB-MP742080VCQ1
CSB-EP742080VCQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Putative arginyl-tRNA--protein transferase (ate) CSB-YP742081VCQ
CSB-EP742081VCQ
CSB-BP742081VCQ
CSB-MP742081VCQ
CSB-EP742081VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Chromosome partition protein mukF (mukF) CSB-YP742082VCQ
CSB-EP742082VCQ
CSB-BP742082VCQ
CSB-MP742082VCQ
CSB-EP742082VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP742083VCQ
CSB-EP742083VCQ
CSB-BP742083VCQ
CSB-MP742083VCQ
CSB-EP742083VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (metE), partial CSB-YP742084VCQ
CSB-EP742084VCQ
CSB-BP742084VCQ
CSB-MP742084VCQ
CSB-EP742084VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus FMN-dependent NADH-azoreductase (azoR) CSB-YP742085VCQ
CSB-EP742085VCQ
CSB-BP742085VCQ
CSB-MP742085VCQ
CSB-EP742085VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Integration host factor subunit alpha (ihfA) CSB-YP742086VCQ
CSB-EP742086VCQ
CSB-BP742086VCQ
CSB-MP742086VCQ
CSB-EP742086VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus 50S ribosomal protein L35 (rpmI) CSB-YP742087VCQ
CSB-EP742087VCQ
CSB-BP742087VCQ
CSB-MP742087VCQ
CSB-EP742087VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Ribosomal RNA large subunit methyltransferase L (rlmL), partial CSB-YP742088VCQ
CSB-EP742088VCQ
CSB-BP742088VCQ
CSB-MP742088VCQ
CSB-EP742088VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus 7-cyano-7-deazaguanine synthase (queC) CSB-YP742089VCQ
CSB-EP742089VCQ
CSB-BP742089VCQ
CSB-MP742089VCQ
CSB-EP742089VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Succinylglutamate desuccinylase (astE) CSB-YP742090VCQ
CSB-EP742090VCQ
CSB-BP742090VCQ
CSB-MP742090VCQ
CSB-EP742090VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Na (+)/H (+) antiporter nhaA (nhaA), partial CSB-YP742091VCQ
CSB-EP742091VCQ
CSB-BP742091VCQ
CSB-MP742091VCQ
CSB-EP742091VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus UPF0225 protein VV1358 (VV1358) CSB-YP742092VCQ
CSB-EP742092VCQ
CSB-BP742092VCQ
CSB-MP742092VCQ
CSB-EP742092VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus ATP phosphoribosyltransferase (hisG) CSB-YP742093VCQ
CSB-EP742093VCQ
CSB-BP742093VCQ
CSB-MP742093VCQ
CSB-EP742093VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Outer-membrane lipoprotein carrier protein (lolA) CSB-YP742094VCQ
CSB-EP742094VCQ
CSB-BP742094VCQ
CSB-MP742094VCQ
CSB-EP742094VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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