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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacillus thuringiensis subsp. konkukian Probable molybdenum cofactor guanylyltransferase (mobA) CSB-YP743496BAAE
CSB-EP743496BAAE
CSB-BP743496BAAE
CSB-MP743496BAAE
CSB-EP743496BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Large-conductance mechanosensitive channel (mscL), partial CSB-YP743497BAAE1
CSB-EP743497BAAE1
CSB-BP743497BAAE1
CSB-MP743497BAAE1
CSB-EP743497BAAE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Argininosuccinate synthase (argG) CSB-YP743498BAAE
CSB-EP743498BAAE
CSB-BP743498BAAE
CSB-MP743498BAAE
CSB-EP743498BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Malate dehydrogenase (mdh) CSB-YP743499BAAE
CSB-EP743499BAAE
CSB-BP743499BAAE
CSB-MP743499BAAE
CSB-EP743499BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Phenylalanine--tRNA ligase beta subunit (pheT), partial CSB-YP743500BAAE
CSB-EP743500BAAE
CSB-BP743500BAAE
CSB-MP743500BAAE
CSB-EP743500BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Cell division protein ZapA (zapA) CSB-YP743501BAAE
CSB-EP743501BAAE
CSB-BP743501BAAE
CSB-MP743501BAAE
CSB-EP743501BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian UvrABC system protein C (uvrC), partial CSB-YP743502BAAE
CSB-EP743502BAAE
CSB-BP743502BAAE
CSB-MP743502BAAE
CSB-EP743502BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Glutamyl-tRNA reductase (hemA) CSB-YP743503BAAE
CSB-EP743503BAAE
CSB-BP743503BAAE
CSB-MP743503BAAE
CSB-EP743503BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian 50S ribosomal protein L21 (rplU) CSB-YP743504BAAE
CSB-EP743504BAAE
CSB-BP743504BAAE
CSB-MP743504BAAE
CSB-EP743504BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Histidine--tRNA ligase 2 (hisS2) CSB-YP743505BAAE
CSB-EP743505BAAE
CSB-BP743505BAAE
CSB-MP743505BAAE
CSB-EP743505BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Germination protease (gpr) CSB-YP743506BAAE
CSB-EP743506BAAE
CSB-BP743506BAAE
CSB-MP743506BAAE
CSB-EP743506BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian L-cystine uptake protein tcyP (BT9727_3988), partial CSB-YP743507BAAE
CSB-EP743507BAAE
CSB-BP743507BAAE
CSB-MP743507BAAE
CSB-EP743507BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian 1-deoxy-D-xylulose-5-phosphate synthase (dxs), partial CSB-YP743508BAAE
CSB-EP743508BAAE
CSB-BP743508BAAE
CSB-MP743508BAAE
CSB-EP743508BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Putative 8-amino-7-oxononanoate synthase (bioF) CSB-YP743509BAAE
CSB-EP743509BAAE
CSB-BP743509BAAE
CSB-MP743509BAAE
CSB-EP743509BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Methylthioribose-1-phosphate isomerase 2 (mtnA2) CSB-YP743510BAAE
CSB-EP743510BAAE
CSB-BP743510BAAE
CSB-MP743510BAAE
CSB-EP743510BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian UPF0358 protein BT9727_3692 (BT9727_3692) CSB-YP743511BAAE
CSB-EP743511BAAE
CSB-BP743511BAAE
CSB-MP743511BAAE
CSB-EP743511BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Uncharacterized N-acetyltransferase BT9727_3663 (BT9727_3663) CSB-YP743512BAAE
CSB-EP743512BAAE
CSB-BP743512BAAE
CSB-MP743512BAAE
CSB-EP743512BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian UDP-N-acetylenolpyruvoylglucosamine reductase 1 (murB1) CSB-YP743513BAAE
CSB-EP743513BAAE
CSB-BP743513BAAE
CSB-MP743513BAAE
CSB-EP743513BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Dihydroorotase (pyrC) CSB-YP743514BAAE
CSB-EP743514BAAE
CSB-BP743514BAAE
CSB-MP743514BAAE
CSB-EP743514BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Orotidine 5'-phosphate decarboxylase (pyrF) CSB-YP743515BAAE
CSB-EP743515BAAE
CSB-BP743515BAAE
CSB-MP743515BAAE
CSB-EP743515BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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