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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacillus thuringiensis subsp. konkukian Pantothenate synthetase (panC) CSB-YP746883BAAE
CSB-EP746883BAAE
CSB-BP746883BAAE
CSB-MP746883BAAE
CSB-EP746883BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Thiol-disulfide oxidoreductase resA (resA), partial CSB-YP746884BAAE1
CSB-EP746884BAAE1
CSB-BP746884BAAE1
CSB-MP746884BAAE1
CSB-EP746884BAAE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Phosphoadenosine phosphosulfate reductase (cysH) CSB-YP746885BAAE
CSB-EP746885BAAE
CSB-BP746885BAAE
CSB-MP746885BAAE
CSB-EP746885BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian 3-isopropylmalate dehydratase small subunit (leuD) CSB-YP746886BAAE
CSB-EP746886BAAE
CSB-BP746886BAAE
CSB-MP746886BAAE
CSB-EP746886BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian UPF0180 protein BT9727_1277 (BT9727_1277) CSB-YP746887BAAE
CSB-EP746887BAAE
CSB-BP746887BAAE
CSB-MP746887BAAE
CSB-EP746887BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian D-alanine--poly (phosphoribitol) ligase subunit 2 (dltC) CSB-YP746888BAAE
CSB-EP746888BAAE
CSB-BP746888BAAE
CSB-MP746888BAAE
CSB-EP746888BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Phosphonoacetaldehyde hydrolase (phnX) CSB-YP746889BAAE
CSB-EP746889BAAE
CSB-BP746889BAAE
CSB-MP746889BAAE
CSB-EP746889BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Lactate utilization protein A (lutA) CSB-YP746890BAAE
CSB-EP746890BAAE
CSB-BP746890BAAE
CSB-MP746890BAAE
CSB-EP746890BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Anthranilate phosphoribosyltransferase (trpD) CSB-YP746891BAAE
CSB-EP746891BAAE
CSB-BP746891BAAE
CSB-MP746891BAAE
CSB-EP746891BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian UPF0736 protein BT9727_1080 (BT9727_1080) CSB-YP746892BAAE
CSB-EP746892BAAE
CSB-BP746892BAAE
CSB-MP746892BAAE
CSB-EP746892BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Undecaprenyl-diphosphatase 2 (uppP2), partial CSB-YP746893BAAE1
CSB-EP746893BAAE1
CSB-BP746893BAAE1
CSB-MP746893BAAE1
CSB-EP746893BAAE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian UPF0374 protein BT9727_0438 (BT9727_0438) CSB-YP746894BAAE
CSB-EP746894BAAE
CSB-BP746894BAAE
CSB-MP746894BAAE
CSB-EP746894BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Proline--tRNA ligase 2 (proS2) CSB-YP746895BAAE
CSB-EP746895BAAE
CSB-BP746895BAAE
CSB-MP746895BAAE
CSB-EP746895BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit C (gatC) CSB-YP746896BAAE
CSB-EP746896BAAE
CSB-BP746896BAAE
CSB-MP746896BAAE
CSB-EP746896BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Phosphoribosylformylglycinamidine synthase 2 (purL), partial CSB-YP746897BAAE
CSB-EP746897BAAE
CSB-BP746897BAAE
CSB-MP746897BAAE
CSB-EP746897BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian 10 kDa chaperonin (groS) CSB-YP746898BAAE
CSB-EP746898BAAE
CSB-BP746898BAAE
CSB-MP746898BAAE
CSB-EP746898BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian DEAD-box ATP-dependent RNA helicase CshA (cshA), partial CSB-YP746899BAAE
CSB-EP746899BAAE
CSB-BP746899BAAE
CSB-MP746899BAAE
CSB-EP746899BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian tRNA pseudouridine synthase A 1 (truA1) CSB-YP746900BAAE
CSB-EP746900BAAE
CSB-BP746900BAAE
CSB-MP746900BAAE
CSB-EP746900BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian 50S ribosomal protein L5 (rplE) CSB-YP746901BAAE
CSB-EP746901BAAE
CSB-BP746901BAAE
CSB-MP746901BAAE
CSB-EP746901BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian 50S ribosomal protein L4 (rplD) CSB-YP746902BAAE
CSB-EP746902BAAE
CSB-BP746902BAAE
CSB-MP746902BAAE
CSB-EP746902BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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