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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Uncharacterized PPE family protein PPE12 (ppe12), partial CSB-YP748523MVZ
CSB-EP748523MVZ
CSB-BP748523MVZ
CSB-MP748523MVZ
CSB-EP748523MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella choleraesuis Quaternary ammonium compound-resistance protein sugE (sugE), partial CSB-YP748524SBF1
CSB-EP748524SBF1
CSB-BP748524SBF1
CSB-MP748524SBF1
CSB-EP748524SBF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Protein grpE (grpE) CSB-YP748525BVW
CSB-EP748525BVW
CSB-BP748525BVW
CSB-MP748525BVW
CSB-EP748525BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum ATP synthase gamma chain (atpG) CSB-YP748526DWY
CSB-EP748526DWY
CSB-BP748526DWY
CSB-MP748526DWY
CSB-EP748526DWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Probable poly-beta-1,6-N-acetyl-D-glucosamine export protein (icaC), partial CSB-YP748527SUL
CSB-EP748527SUL
CSB-BP748527SUL
CSB-MP748527SUL
CSB-EP748527SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Staphylococcal secretory antigen ssaA1 (ssaA1) CSB-YP748528SUL
CSB-EP748528SUL
CSB-BP748528SUL
CSB-MP748528SUL
CSB-EP748528SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Lysostaphin resistance protein A (lyrA), partial CSB-YP748529SUL
CSB-EP748529SUL
CSB-BP748529SUL
CSB-MP748529SUL
CSB-EP748529SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 50S ribosomal protein L16 (rplP) CSB-YP748530SUL
CSB-EP748530SUL
CSB-BP748530SUL
CSB-MP748530SUL
CSB-EP748530SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Energy-coupling factor transporter ATP-binding protein EcfA 1 (ecfA1) CSB-YP748531SUL
CSB-EP748531SUL
CSB-BP748531SUL
CSB-MP748531SUL
CSB-EP748531SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus ATP synthase subunit c (atpE), partial CSB-YP748532SUL1
CSB-EP748532SUL1
CSB-BP748532SUL1
CSB-MP748532SUL1
CSB-EP748532SUL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus UPF0435 protein MW1820 (MW1820) CSB-YP748533SUL
CSB-EP748533SUL
CSB-BP748533SUL
CSB-MP748533SUL
CSB-EP748533SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Tyrosine--tRNA ligase (tyrS) CSB-YP748534SUL
CSB-EP748534SUL
CSB-BP748534SUL
CSB-MP748534SUL
CSB-EP748534SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus UPF0473 protein MW1565 (MW1565) CSB-YP748535SUL
CSB-EP748535SUL
CSB-BP748535SUL
CSB-MP748535SUL
CSB-EP748535SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Cell cycle protein GpsB (gpsB) CSB-YP748536SUL
CSB-EP748536SUL
CSB-BP748536SUL
CSB-MP748536SUL
CSB-EP748536SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Cold shock protein CspA (cspA) CSB-YP748537SUL
CSB-EP748537SUL
CSB-BP748537SUL
CSB-MP748537SUL
CSB-EP748537SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Uncharacterized protein MW1239 (MW1239) CSB-YP748538SUL
CSB-EP748538SUL
CSB-BP748538SUL
CSB-MP748538SUL
CSB-EP748538SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Phosphatidate cytidylyltransferase (cdsA), partial CSB-YP748539SUL1
CSB-EP748539SUL1
CSB-BP748539SUL1
CSB-MP748539SUL1
CSB-EP748539SUL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Iron-regulated surface determinant protein C (isdC) CSB-YP748540SUL
CSB-EP748540SUL
CSB-BP748540SUL
CSB-MP748540SUL
CSB-EP748540SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Probable quinol oxidase subunit 1 (qoxB), partial CSB-YP748541SUL
CSB-EP748541SUL
CSB-BP748541SUL
CSB-MP748541SUL
CSB-EP748541SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus NAD-specific glutamate dehydrogenase (gluD) CSB-YP748542SUL
CSB-EP748542SUL
CSB-BP748542SUL
CSB-MP748542SUL
CSB-EP748542SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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