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重組蛋白

產品名稱 貨號 規(guī)格
Recombinant Bacillus thuringiensis subsp. konkukian UPF0176 protein BT9727_1719 (BT9727_1719) CSB-YP753655BAAE
CSB-EP753655BAAE
CSB-BP753655BAAE
CSB-MP753655BAAE
CSB-EP753655BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Aspartate 1-decarboxylase (panD) CSB-YP753656BAAE
CSB-EP753656BAAE
CSB-BP753656BAAE
CSB-MP753656BAAE
CSB-EP753656BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian CCA-adding enzyme (cca) CSB-YP753657BAAE
CSB-EP753657BAAE
CSB-BP753657BAAE
CSB-MP753657BAAE
CSB-EP753657BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Phosphoribosyl-ATP pyrophosphatase (hisE) CSB-YP753658BAAE
CSB-EP753658BAAE
CSB-BP753658BAAE
CSB-MP753658BAAE
CSB-EP753658BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian 1- (5-phosphoribosyl)-5-[ (5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase (hisA) CSB-YP753659BAAE
CSB-EP753659BAAE
CSB-BP753659BAAE
CSB-MP753659BAAE
CSB-EP753659BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian 3-isopropylmalate dehydrogenase (leuB) CSB-YP753660BAAE
CSB-EP753660BAAE
CSB-BP753660BAAE
CSB-MP753660BAAE
CSB-EP753660BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Probable multidrug resistance protein norM (norM), partial CSB-YP753661BAAE
CSB-EP753661BAAE
CSB-BP753661BAAE
CSB-MP753661BAAE
CSB-EP753661BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Lactate utilization protein C (lutC) CSB-YP753662BAAE
CSB-EP753662BAAE
CSB-BP753662BAAE
CSB-MP753662BAAE
CSB-EP753662BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Tryptophan synthase alpha chain (trpA) CSB-YP753663BAAE
CSB-EP753663BAAE
CSB-BP753663BAAE
CSB-MP753663BAAE
CSB-EP753663BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Bis (5'-nucleosyl)-tetraphosphatase prpE [asymmetrical] (prpE) CSB-YP753664BAAE
CSB-EP753664BAAE
CSB-BP753664BAAE
CSB-MP753664BAAE
CSB-EP753664BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian 3'-5' exoribonuclease yhaM (yhaM) CSB-YP753665BAAE
CSB-EP753665BAAE
CSB-BP753665BAAE
CSB-MP753665BAAE
CSB-EP753665BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Probable DNA repair protein BT9727_0719 (BT9727_0719) CSB-YP753666BAAE
CSB-EP753666BAAE
CSB-BP753666BAAE
CSB-MP753666BAAE
CSB-EP753666BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Probable transaldolase (tal) CSB-YP753667BAAE
CSB-EP753667BAAE
CSB-BP753667BAAE
CSB-MP753667BAAE
CSB-EP753667BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Probable transcriptional regulatory protein BT9727_0453 (BT9727_0453) CSB-YP753668BAAE
CSB-EP753668BAAE
CSB-BP753668BAAE
CSB-MP753668BAAE
CSB-EP753668BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Probable methyl-accepting chemotaxis protein BT9727_0355 (BT9727_0355), partial CSB-YP753669BAAE
CSB-EP753669BAAE
CSB-BP753669BAAE
CSB-MP753669BAAE
CSB-EP753669BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Arginine deiminase (arcA) CSB-YP753670BAAE
CSB-EP753670BAAE
CSB-BP753670BAAE
CSB-MP753670BAAE
CSB-EP753670BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Glutamyl-tRNA (Gln) amidotransferase subunit A (gatA) CSB-YP753671BAAE
CSB-EP753671BAAE
CSB-BP753671BAAE
CSB-MP753671BAAE
CSB-EP753671BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian 60 kDa chaperonin (groL), partial CSB-YP753672BAAE
CSB-EP753672BAAE
CSB-BP753672BAAE
CSB-MP753672BAAE
CSB-EP753672BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Protein sprT-like (BT9727_0228) CSB-YP753673BAAE
CSB-EP753673BAAE
CSB-BP753673BAAE
CSB-MP753673BAAE
CSB-EP753673BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Phosphoglucosamine mutase (glmM) CSB-YP753674BAAE
CSB-EP753674BAAE
CSB-BP753674BAAE
CSB-MP753674BAAE
CSB-EP753674BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產生大量蛋白質、修改基因序列和制造有用的商業(yè)產品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產,在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們如何確保蛋白質的產生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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