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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Ashbya gossypii Glutamyl-tRNA (Gln) amidotransferase subunit A, mitochondrial (HER2) CSB-YP755178DOT
CSB-EP755178DOT
CSB-BP755178DOT
CSB-MP755178DOT
CSB-EP755178DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Chromosome segregation in meiosis protein 3 (CSM3) CSB-YP755179DOT
CSB-EP755179DOT
CSB-BP755179DOT
CSB-MP755179DOT
CSB-EP755179DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Mannan endo-1,6-alpha-mannosidase DCW1 (DCW1) CSB-YP755180DOT
CSB-EP755180DOT
CSB-BP755180DOT
CSB-MP755180DOT
CSB-EP755180DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii ATPase synthesis protein 25, mitochondrial (ATP25), partial CSB-YP755181DOT
CSB-EP755181DOT
CSB-BP755181DOT
CSB-MP755181DOT
CSB-EP755181DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Pre-rRNA-processing protein RIX1 (RIX1), partial CSB-YP755182DOT
CSB-EP755182DOT
CSB-BP755182DOT
CSB-MP755182DOT
CSB-EP755182DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Riboflavin kinase (FMN1) CSB-YP755183DOT
CSB-EP755183DOT
CSB-BP755183DOT
CSB-MP755183DOT
CSB-EP755183DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Heat shock protein SSB (SSB), partial CSB-YP755184DOT
CSB-EP755184DOT
CSB-BP755184DOT
CSB-MP755184DOT
CSB-EP755184DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Cytosolic Fe-S cluster assembly factor NAR1 (NAR1) CSB-YP755185DOT
CSB-EP755185DOT
CSB-BP755185DOT
CSB-MP755185DOT
CSB-EP755185DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Ubiquitin-conjugating enzyme E2 2 (UBC2) CSB-YP755186DOT
CSB-EP755186DOT
CSB-BP755186DOT
CSB-MP755186DOT
CSB-EP755186DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Vacuolar ATPase assembly integral membrane protein VMA21 (VMA21), partial CSB-YP755187DOT
CSB-EP755187DOT
CSB-BP755187DOT
CSB-MP755187DOT
CSB-EP755187DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Copper transport protein 86 (CTR86), partial CSB-YP755188DOT
CSB-EP755188DOT
CSB-BP755188DOT
CSB-MP755188DOT
CSB-EP755188DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Mitochondrial thiamine pyrophosphate carrier 1 (TPC1), partial CSB-YP755189DOT1
CSB-EP755189DOT1
CSB-BP755189DOT1
CSB-MP755189DOT1
CSB-EP755189DOT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Mediator of RNA polymerase II transcription subunit 21 (SRB7) CSB-YP755190DOT
CSB-EP755190DOT
CSB-BP755190DOT
CSB-MP755190DOT
CSB-EP755190DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Protein BFR2 (BFR2), partial CSB-YP755191DOT
CSB-EP755191DOT
CSB-BP755191DOT
CSB-MP755191DOT
CSB-EP755191DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Autophagy-related protein 29 (ATG29) CSB-YP755192DOT
CSB-EP755192DOT
CSB-BP755192DOT
CSB-MP755192DOT
CSB-EP755192DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Mitochondrial import inner membrane translocase subunit TIM13 (TIM13) CSB-YP755193DOT
CSB-EP755193DOT
CSB-BP755193DOT
CSB-MP755193DOT
CSB-EP755193DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni Acireductone dioxygenase (mtnD) CSB-YP755194LEV
CSB-EP755194LEV
CSB-BP755194LEV
CSB-MP755194LEV
CSB-EP755194LEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni Acetylglutamate kinase (argB) CSB-YP755195LEV
CSB-EP755195LEV
CSB-BP755195LEV
CSB-MP755195LEV
CSB-EP755195LEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii ATP synthase subunit a (ATP6), partial CSB-YP755196DOT1
CSB-EP755196DOT1
CSB-BP755196DOT1
CSB-MP755196DOT1
CSB-EP755196DOT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Auxin-responsive protein IAA11 (IAA11) CSB-YP755197OFG
CSB-EP755197OFG
CSB-BP755197OFG
CSB-MP755197OFG
CSB-EP755197OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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