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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Ashbya gossypii Ubiquinone biosynthesis protein COQ4, mitochondrial (COQ4), partial CSB-YP758698DOT
CSB-EP758698DOT
CSB-BP758698DOT
CSB-MP758698DOT
CSB-EP758698DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii SAGA-associated factor 11 (SGF11) CSB-YP758699DOT
CSB-EP758699DOT
CSB-BP758699DOT
CSB-MP758699DOT
CSB-EP758699DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Serine/threonine-protein kinase TEL1 (TEL1), partial CSB-YP758700DOT
CSB-EP758700DOT
CSB-BP758700DOT
CSB-MP758700DOT
CSB-EP758700DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Cytoplasmic dynein intermediate light chain DYN3 (DYN3) CSB-YP758701DOT
CSB-EP758701DOT
CSB-BP758701DOT
CSB-MP758701DOT
CSB-EP758701DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii AdoMet-dependent rRNA methyltransferase SPB1 (SPB1), partial CSB-YP758702DOT
CSB-EP758702DOT
CSB-BP758702DOT
CSB-MP758702DOT
CSB-EP758702DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Plasma membrane fusion protein PRM1 (PRM1), partial CSB-YP758703DOT1
CSB-EP758703DOT1
CSB-BP758703DOT1
CSB-MP758703DOT1
CSB-EP758703DOT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Protein SOP4 (SOP4), partial CSB-YP758704DOT
CSB-EP758704DOT
CSB-BP758704DOT
CSB-MP758704DOT
CSB-EP758704DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Pheromone-processing carboxypeptidase KEX1 (KEX1), partial CSB-YP758705DOT
CSB-EP758705DOT
CSB-BP758705DOT
CSB-MP758705DOT
CSB-EP758705DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Mediator of RNA polymerase II transcription subunit 3 (PGD1) CSB-YP758706DOT
CSB-EP758706DOT
CSB-BP758706DOT
CSB-MP758706DOT
CSB-EP758706DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Tubulin alpha chain (TUB1) CSB-YP758707DOT
CSB-EP758707DOT
CSB-BP758707DOT
CSB-MP758707DOT
CSB-EP758707DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Pre-mRNA-splicing factor SPP2 (SPP2) CSB-YP758708DOT
CSB-EP758708DOT
CSB-BP758708DOT
CSB-MP758708DOT
CSB-EP758708DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Cytochrome c (CYC1) CSB-YP758709DOT
CSB-EP758709DOT
CSB-BP758709DOT
CSB-MP758709DOT
CSB-EP758709DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Suppressor of lethality of KEX2 GAS1 double null mutant protein 1 (SKG1), partial CSB-YP758710DOT
CSB-EP758710DOT
CSB-BP758710DOT
CSB-MP758710DOT
CSB-EP758710DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Sensitivity to high expression protein 10 (SHE10), partial CSB-YP758711DOT
CSB-EP758711DOT
CSB-BP758711DOT
CSB-MP758711DOT
CSB-EP758711DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii DASH complex subunit HSK3 (HSK3) CSB-YP758712DOT
CSB-EP758712DOT
CSB-BP758712DOT
CSB-MP758712DOT
CSB-EP758712DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii C-5 sterol desaturase (ERG3), partial CSB-YP758713DOT1
CSB-EP758713DOT1
CSB-BP758713DOT1
CSB-MP758713DOT1
CSB-EP758713DOT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Actin-related protein 4 (ARP4) CSB-YP758714DOT
CSB-EP758714DOT
CSB-BP758714DOT
CSB-MP758714DOT
CSB-EP758714DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Mitochondrial import inner membrane translocase subunit TIM16 (PAM16) CSB-YP758715DOT
CSB-EP758715DOT
CSB-BP758715DOT
CSB-MP758715DOT
CSB-EP758715DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Oligosaccharide translocation protein RFT1 (RFT1), partial CSB-YP758716DOT
CSB-EP758716DOT
CSB-BP758716DOT
CSB-MP758716DOT
CSB-EP758716DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii DASH complex subunit DAD3 (DAD3) CSB-YP758717DOT
CSB-EP758717DOT
CSB-BP758717DOT
CSB-MP758717DOT
CSB-EP758717DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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