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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Vibrio vulnificus Ribonuclease HII (rnhB) CSB-YP766079VCQ
CSB-EP766079VCQ
CSB-BP766079VCQ
CSB-MP766079VCQ
CSB-EP766079VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Succinyl-diaminopimelate desuccinylase (dapE) CSB-YP766080VCQ
CSB-EP766080VCQ
CSB-BP766080VCQ
CSB-MP766080VCQ
CSB-EP766080VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus tRNA 5-methylaminomethyl-2-thiouridine biosynthesis bifunctional protein MnmC (mnmC), partial CSB-YP766081VCQ
CSB-EP766081VCQ
CSB-BP766081VCQ
CSB-MP766081VCQ
CSB-EP766081VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus UPF0260 protein VV2402 (VV2402) CSB-YP766082VCQ
CSB-EP766082VCQ
CSB-BP766082VCQ
CSB-MP766082VCQ
CSB-EP766082VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus UPF0229 protein VV2350 (VV2350) CSB-YP766083VCQ
CSB-EP766083VCQ
CSB-BP766083VCQ
CSB-MP766083VCQ
CSB-EP766083VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Glucose-1-phosphate adenylyltransferase 1 (glgC1) CSB-YP766084VCQ
CSB-EP766084VCQ
CSB-BP766084VCQ
CSB-MP766084VCQ
CSB-EP766084VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus tRNA (cmo5U34)-methyltransferase (cmoA) CSB-YP766085VCQ
CSB-EP766085VCQ
CSB-BP766085VCQ
CSB-MP766085VCQ
CSB-EP766085VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus NAD-dependent malic enzyme 2 (maeA2), partial CSB-YP766086VCQ
CSB-EP766086VCQ
CSB-BP766086VCQ
CSB-MP766086VCQ
CSB-EP766086VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Na (+)/H (+) antiporter nhaB (nhaB), partial CSB-YP766087VCQ
CSB-EP766087VCQ
CSB-BP766087VCQ
CSB-MP766087VCQ
CSB-EP766087VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Phenylalanine--tRNA ligase alpha subunit (pheS) CSB-YP766088VCQ
CSB-EP766088VCQ
CSB-BP766088VCQ
CSB-MP766088VCQ
CSB-EP766088VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus tRNA 2-thiocytidine biosynthesis protein TtcA (ttcA) CSB-YP766089VCQ
CSB-EP766089VCQ
CSB-BP766089VCQ
CSB-MP766089VCQ
CSB-EP766089VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Acylphosphatase (acyP) CSB-YP766090VCQ
CSB-EP766090VCQ
CSB-BP766090VCQ
CSB-MP766090VCQ
CSB-EP766090VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Vitamin B12 import system permease protein BtuC (btuC), partial CSB-YP766091VCQ
CSB-EP766091VCQ
CSB-BP766091VCQ
CSB-MP766091VCQ
CSB-EP766091VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Putative transport protein VV1438 (VV1438), partial CSB-YP766092VCQ
CSB-EP766092VCQ
CSB-BP766092VCQ
CSB-MP766092VCQ
CSB-EP766092VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Imidazole glycerol phosphate synthase subunit HisH 1 (hisH1) CSB-YP766093VCQ
CSB-EP766093VCQ
CSB-BP766093VCQ
CSB-MP766093VCQ
CSB-EP766093VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus ATP-dependent dethiobiotin synthetase BioD (bioD) CSB-YP766094VCQ
CSB-EP766094VCQ
CSB-BP766094VCQ
CSB-MP766094VCQ
CSB-EP766094VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Phosphate acyltransferase (plsX) CSB-YP766095VCQ
CSB-EP766095VCQ
CSB-BP766095VCQ
CSB-MP766095VCQ
CSB-EP766095VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus UPF0263 protein VV1228 (VV1228) CSB-YP766096VCQ
CSB-EP766096VCQ
CSB-BP766096VCQ
CSB-MP766096VCQ
CSB-EP766096VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Molybdenum cofactor biosynthesis protein C (moaC) CSB-YP766097VCQ
CSB-EP766097VCQ
CSB-BP766097VCQ
CSB-MP766097VCQ
CSB-EP766097VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Electron transport complex protein RnfD (rnfD), partial CSB-YP766098VCQ
CSB-EP766098VCQ
CSB-BP766098VCQ
CSB-MP766098VCQ
CSB-EP766098VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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