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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Xylella fastidiosa 50S ribosomal protein L10 (rplJ) CSB-YP769838XAT
CSB-EP769838XAT
CSB-BP769838XAT
CSB-MP769838XAT
CSB-EP769838XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Putative pterin-4-alpha-carbinolamine dehydratase (PD_1980) CSB-YP769839XAT
CSB-EP769839XAT
CSB-BP769839XAT
CSB-MP769839XAT
CSB-EP769839XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (ispG) CSB-YP769840XAT
CSB-EP769840XAT
CSB-BP769840XAT
CSB-MP769840XAT
CSB-EP769840XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Methylthioribose-1-phosphate isomerase (mtnA) CSB-YP769841XAT
CSB-EP769841XAT
CSB-BP769841XAT
CSB-MP769841XAT
CSB-EP769841XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa 50S ribosomal protein L20 (rplT) CSB-YP769842XAT
CSB-EP769842XAT
CSB-BP769842XAT
CSB-MP769842XAT
CSB-EP769842XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Exodeoxyribonuclease 7 large subunit (xseA) CSB-YP769843XAT
CSB-EP769843XAT
CSB-BP769843XAT
CSB-MP769843XAT
CSB-EP769843XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Beta-lactamase hydrolase-like protein (blh) CSB-YP769844XAT
CSB-EP769844XAT
CSB-BP769844XAT
CSB-MP769844XAT
CSB-EP769844XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY), partial CSB-YP769845XAT
CSB-EP769845XAT
CSB-BP769845XAT
CSB-MP769845XAT
CSB-EP769845XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Glutamate--tRNA ligase (gltX) CSB-YP769846XAT
CSB-EP769846XAT
CSB-BP769846XAT
CSB-MP769846XAT
CSB-EP769846XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa D-amino acid dehydrogenase small subunit (dadA) CSB-YP769847XAT
CSB-EP769847XAT
CSB-BP769847XAT
CSB-MP769847XAT
CSB-EP769847XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Methionyl-tRNA formyltransferase (fmt) CSB-YP769848XAT
CSB-EP769848XAT
CSB-BP769848XAT
CSB-MP769848XAT
CSB-EP769848XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Laccase domain protein PD_1754 (PD_1754) CSB-YP769849XAT
CSB-EP769849XAT
CSB-BP769849XAT
CSB-MP769849XAT
CSB-EP769849XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa N utilization substance protein B homolog (nusB) CSB-YP769850XAT
CSB-EP769850XAT
CSB-BP769850XAT
CSB-MP769850XAT
CSB-EP769850XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Thiamine-phosphate synthase (thiE) CSB-YP769851XAT
CSB-EP769851XAT
CSB-BP769851XAT
CSB-MP769851XAT
CSB-EP769851XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Tryptophan--tRNA ligase (trpS) CSB-YP769852XAT
CSB-EP769852XAT
CSB-BP769852XAT
CSB-MP769852XAT
CSB-EP769852XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa GTPase Der (der) CSB-YP769853XAT
CSB-EP769853XAT
CSB-BP769853XAT
CSB-MP769853XAT
CSB-EP769853XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa tRNA 2-thiocytidine biosynthesis protein TtcA (ttcA) CSB-YP769854XAT
CSB-EP769854XAT
CSB-BP769854XAT
CSB-MP769854XAT
CSB-EP769854XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa tRNA (Ile)-lysidine synthase (tilS) CSB-YP769855XAT
CSB-EP769855XAT
CSB-BP769855XAT
CSB-MP769855XAT
CSB-EP769855XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Homoserine O-acetyltransferase (metX) CSB-YP769856XAT
CSB-EP769856XAT
CSB-BP769856XAT
CSB-MP769856XAT
CSB-EP769856XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Segregation and condensation protein A (scpA) CSB-YP769857XAT
CSB-EP769857XAT
CSB-BP769857XAT
CSB-MP769857XAT
CSB-EP769857XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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