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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Xylella fastidiosa 50S ribosomal protein L4 (rplD) CSB-YP771555XAT
CSB-EP771555XAT
CSB-BP771555XAT
CSB-MP771555XAT
CSB-EP771555XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa ATP synthase epsilon chain (atpC) CSB-YP771556XAT
CSB-EP771556XAT
CSB-BP771556XAT
CSB-MP771556XAT
CSB-EP771556XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Nicotinate phosphoribosyltransferase (pncB) CSB-YP771557XAT
CSB-EP771557XAT
CSB-BP771557XAT
CSB-MP771557XAT
CSB-EP771557XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Glucokinase (glk) CSB-YP771558XAT
CSB-EP771558XAT
CSB-BP771558XAT
CSB-MP771558XAT
CSB-EP771558XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Lipid-A-disaccharide synthase (lpxB) CSB-YP771559XAT
CSB-EP771559XAT
CSB-BP771559XAT
CSB-MP771559XAT
CSB-EP771559XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Glutamate 5-kinase (proB) CSB-YP771560XAT
CSB-EP771560XAT
CSB-BP771560XAT
CSB-MP771560XAT
CSB-EP771560XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Phosphopantetheine adenylyltransferase (coaD) CSB-YP771561XAT
CSB-EP771561XAT
CSB-BP771561XAT
CSB-MP771561XAT
CSB-EP771561XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa NADH-quinone oxidoreductase subunit B (nuoB) CSB-YP771562XAT
CSB-EP771562XAT
CSB-BP771562XAT
CSB-MP771562XAT
CSB-EP771562XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Ribosome-binding factor A (rbfA) CSB-YP771563XAT
CSB-EP771563XAT
CSB-BP771563XAT
CSB-MP771563XAT
CSB-EP771563XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Indole-3-glycerol phosphate synthase (trpC) CSB-YP771564XAT
CSB-EP771564XAT
CSB-BP771564XAT
CSB-MP771564XAT
CSB-EP771564XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Glycine cleavage system H protein (gcvH) CSB-YP771565XAT
CSB-EP771565XAT
CSB-BP771565XAT
CSB-MP771565XAT
CSB-EP771565XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Biosynthetic arginine decarboxylase (speA), partial CSB-YP771566XAT
CSB-EP771566XAT
CSB-BP771566XAT
CSB-MP771566XAT
CSB-EP771566XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Protein RecA (recA) CSB-YP771567XAT
CSB-EP771567XAT
CSB-BP771567XAT
CSB-MP771567XAT
CSB-EP771567XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Dihydroxy-acid dehydratase (ilvD), partial CSB-YP771568XAT
CSB-EP771568XAT
CSB-BP771568XAT
CSB-MP771568XAT
CSB-EP771568XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Flavohemoprotein (hmp) CSB-YP771569XAT
CSB-EP771569XAT
CSB-BP771569XAT
CSB-MP771569XAT
CSB-EP771569XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Chromosomal replication initiator protein DnaA (dnaA) CSB-YP771570XAT
CSB-EP771570XAT
CSB-BP771570XAT
CSB-MP771570XAT
CSB-EP771570XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant L-arabinose isomerase (araA) CSB-YP771571VFE
CSB-EP771571VFE
CSB-BP771571VFE
CSB-MP771571VFE
CSB-EP771571VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serine/threonine transporter sstT (sstT), partial CSB-YP771572VFE
CSB-EP771572VFE
CSB-BP771572VFE
CSB-MP771572VFE
CSB-EP771572VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant L-lactate dehydrogenase [cytochrome] (lldD) CSB-YP771573VFE
CSB-EP771573VFE
CSB-BP771573VFE
CSB-MP771573VFE
CSB-EP771573VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Maltose transport system permease protein malF (malF), partial CSB-YP771574VFE
CSB-EP771574VFE
CSB-BP771574VFE
CSB-MP771574VFE
CSB-EP771574VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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