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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Staphylococcus epidermidis GTPase obg (obg) CSB-YP807567SLD
CSB-EP807567SLD
CSB-BP807567SLD
CSB-MP807567SLD
CSB-EP807567SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis DNA repair protein recO (recO) CSB-YP807568SLD
CSB-EP807568SLD
CSB-BP807568SLD
CSB-MP807568SLD
CSB-EP807568SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Arginine repressor (argR) CSB-YP807569SLD
CSB-EP807569SLD
CSB-BP807569SLD
CSB-MP807569SLD
CSB-EP807569SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Probable ATP-dependent helicase dinG homolog (dinG), partial CSB-YP807570SLD
CSB-EP807570SLD
CSB-BP807570SLD
CSB-MP807570SLD
CSB-EP807570SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Putative branched-chain amino acid carrier protein SE_1090 (SE_1090), partial CSB-YP807571SLD
CSB-EP807571SLD
CSB-BP807571SLD
CSB-MP807571SLD
CSB-EP807571SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Tryptophan synthase beta chain (trpB) CSB-YP807572SLD
CSB-EP807572SLD
CSB-BP807572SLD
CSB-MP807572SLD
CSB-EP807572SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Aconitate hydratase (acnA), partial CSB-YP807573SLD
CSB-EP807573SLD
CSB-BP807573SLD
CSB-MP807573SLD
CSB-EP807573SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis DNA mismatch repair protein MutS (mutS), partial CSB-YP807574SLD
CSB-EP807574SLD
CSB-BP807574SLD
CSB-MP807574SLD
CSB-EP807574SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Elongation factor Ts (tsf) CSB-YP807575SLD
CSB-EP807575SLD
CSB-BP807575SLD
CSB-MP807575SLD
CSB-EP807575SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Succinyl-CoA ligase [ADP-forming] subunit alpha (sucD) CSB-YP807576SLD
CSB-EP807576SLD
CSB-BP807576SLD
CSB-MP807576SLD
CSB-EP807576SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Carbamoyl-phosphate synthase large chain (carB), partial CSB-YP807577SLD
CSB-EP807577SLD
CSB-BP807577SLD
CSB-MP807577SLD
CSB-EP807577SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY), partial CSB-YP807578SLD
CSB-EP807578SLD
CSB-BP807578SLD
CSB-MP807578SLD
CSB-EP807578SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Pyruvate dehydrogenase E1 component subunit beta (pdhB) CSB-YP807579SLD
CSB-EP807579SLD
CSB-BP807579SLD
CSB-MP807579SLD
CSB-EP807579SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Probable quinol oxidase subunit 3 (qoxC), partial CSB-YP807580SLD1
CSB-EP807580SLD1
CSB-BP807580SLD1
CSB-MP807580SLD1
CSB-EP807580SLD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis 3-oxoacyl-[acyl-carrier-protein] synthase 3 protein 1 (fabH1) CSB-YP807581SLD
CSB-EP807581SLD
CSB-BP807581SLD
CSB-MP807581SLD
CSB-EP807581SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Na (+)/H (+) antiporter subunit B1 (mnhB1), partial CSB-YP807582SLD1
CSB-EP807582SLD1
CSB-BP807582SLD1
CSB-MP807582SLD1
CSB-EP807582SLD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Protein nagD homolog (nagD) CSB-YP807583SLD
CSB-EP807583SLD
CSB-BP807583SLD
CSB-MP807583SLD
CSB-EP807583SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (gpmI), partial CSB-YP807584SLD
CSB-EP807584SLD
CSB-BP807584SLD
CSB-MP807584SLD
CSB-EP807584SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Response regulator saeR (saeR) CSB-YP807585SLD
CSB-EP807585SLD
CSB-BP807585SLD
CSB-MP807585SLD
CSB-EP807585SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Putative antiporter subunit mnhA2 (mnhA2), partial CSB-YP807586SLD
CSB-EP807586SLD
CSB-BP807586SLD
CSB-MP807586SLD
CSB-EP807586SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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