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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Staphylococcus epidermidis CCA-adding enzyme (cca) CSB-YP811910SLD
CSB-EP811910SLD
CSB-BP811910SLD
CSB-MP811910SLD
CSB-EP811910SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Dihydrodipicolinate synthase (dapA) CSB-YP811911SLD
CSB-EP811911SLD
CSB-BP811911SLD
CSB-MP811911SLD
CSB-EP811911SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Tryptophan synthase alpha chain (trpA) CSB-YP811912SLD
CSB-EP811912SLD
CSB-BP811912SLD
CSB-MP811912SLD
CSB-EP811912SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Nuclease sbcCD subunit D (sbcD) CSB-YP811913SLD
CSB-EP811913SLD
CSB-BP811913SLD
CSB-MP811913SLD
CSB-EP811913SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Aerobic glycerol-3-phosphate dehydrogenase (glpD), partial CSB-YP811914SLD
CSB-EP811914SLD
CSB-BP811914SLD
CSB-MP811914SLD
CSB-EP811914SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Succinyl-CoA ligase [ADP-forming] subunit beta (sucC) CSB-YP811915SLD
CSB-EP811915SLD
CSB-BP811915SLD
CSB-MP811915SLD
CSB-EP811915SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Peptide deformylase-like (SE_0890) CSB-YP811916SLD
CSB-EP811916SLD
CSB-BP811916SLD
CSB-MP811916SLD
CSB-EP811916SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Bifunctional protein pyrR (pyrR) CSB-YP811917SLD
CSB-EP811917SLD
CSB-BP811917SLD
CSB-MP811917SLD
CSB-EP811917SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis UvrABC system protein C (uvrC), partial CSB-YP811918SLD
CSB-EP811918SLD
CSB-BP811918SLD
CSB-MP811918SLD
CSB-EP811918SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Heme A synthase (ctaA), partial CSB-YP811919SLD1
CSB-EP811919SLD1
CSB-BP811919SLD1
CSB-MP811919SLD1
CSB-EP811919SLD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Bifunctional protein FolD (folD) CSB-YP811920SLD
CSB-EP811920SLD
CSB-BP811920SLD
CSB-MP811920SLD
CSB-EP811920SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis 1,4-Dihydroxy-2-naphthoyl-CoA synthase (menB) CSB-YP811921SLD
CSB-EP811921SLD
CSB-BP811921SLD
CSB-MP811921SLD
CSB-EP811921SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Uncharacterized protein SE_0665 (SE_0665) CSB-YP811922SLD
CSB-EP811922SLD
CSB-BP811922SLD
CSB-MP811922SLD
CSB-EP811922SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Na (+)/H (+) antiporter subunit A1 (mnhA1), partial CSB-YP811923SLD
CSB-EP811923SLD
CSB-BP811923SLD
CSB-MP811923SLD
CSB-EP811923SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis D-alanine--poly (phosphoribitol) ligase subunit 2 (dltC) CSB-YP811924SLD
CSB-EP811924SLD
CSB-BP811924SLD
CSB-MP811924SLD
CSB-EP811924SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Probable protein-export membrane protein SecG (secG), partial CSB-YP811925SLD1
CSB-EP811925SLD1
CSB-BP811925SLD1
CSB-MP811925SLD1
CSB-EP811925SLD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Peptide chain release factor 2 (prfB) CSB-YP811926SLD
CSB-EP811926SLD
CSB-BP811926SLD
CSB-MP811926SLD
CSB-EP811926SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Putative lipid kinase SE_0507 (SE_0507) CSB-YP811927SLD
CSB-EP811927SLD
CSB-BP811927SLD
CSB-MP811927SLD
CSB-EP811927SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Putative antiporter subunit mnhD2 (mnhD2), partial CSB-YP811928SLD
CSB-EP811928SLD
CSB-BP811928SLD
CSB-MP811928SLD
CSB-EP811928SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Uncharacterized epimerase/dehydratase SE_0317 (SE_0317) CSB-YP811929SLD
CSB-EP811929SLD
CSB-BP811929SLD
CSB-MP811929SLD
CSB-EP811929SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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