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重組蛋白

產品名稱 貨號 規(guī)格
Recombinant Shewanella oneidensis Ribosomal protein S12 methylthiotransferase RimO (rimO) CSB-YP812331SGA
CSB-EP812331SGA
CSB-BP812331SGA
CSB-MP812331SGA
CSB-EP812331SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis UDP-N-acetylglucosamine 1-carboxyvinyltransferase (murA) CSB-YP812332SGA
CSB-EP812332SGA
CSB-BP812332SGA
CSB-MP812332SGA
CSB-EP812332SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis 23S rRNA (guanosine-2'-O-)-methyltransferase RlmB (rlmB) CSB-YP812333SGA
CSB-EP812333SGA
CSB-BP812333SGA
CSB-MP812333SGA
CSB-EP812333SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis Putative protein-disulfide oxidoreductase (SO_3870), partial CSB-YP812334SGA1
CSB-EP812334SGA1
CSB-BP812334SGA1
CSB-MP812334SGA1
CSB-EP812334SGA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis 2-dehydro-3-deoxyphosphooctonate aldolase (kdsA) CSB-YP812335SGA
CSB-EP812335SGA
CSB-BP812335SGA
CSB-MP812335SGA
CSB-EP812335SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis Sulfate adenylyltransferase subunit 2 (cysD) CSB-YP812336SGA
CSB-EP812336SGA
CSB-BP812336SGA
CSB-MP812336SGA
CSB-EP812336SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis Chaperone surA (surA) CSB-YP812337SGA
CSB-EP812337SGA
CSB-BP812337SGA
CSB-MP812337SGA
CSB-EP812337SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis Glucose-6-phosphate isomerase (pgi), partial CSB-YP812338SGA
CSB-EP812338SGA
CSB-BP812338SGA
CSB-MP812338SGA
CSB-EP812338SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH) CSB-YP812339SGA
CSB-EP812339SGA
CSB-BP812339SGA
CSB-MP812339SGA
CSB-EP812339SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis Alanine--tRNA ligase (alaS), partial CSB-YP812340SGA
CSB-EP812340SGA
CSB-BP812340SGA
CSB-MP812340SGA
CSB-EP812340SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis tRNA (guanine-N (7)-)-methyltransferase (trmB) CSB-YP812341SGA
CSB-EP812341SGA
CSB-BP812341SGA
CSB-MP812341SGA
CSB-EP812341SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis UPF0301 protein SO_3346 (SO_3346) CSB-YP812342SGA
CSB-EP812342SGA
CSB-BP812342SGA
CSB-MP812342SGA
CSB-EP812342SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis Flagellar L-ring protein (flgH) CSB-YP812343SGA
CSB-EP812343SGA
CSB-BP812343SGA
CSB-MP812343SGA
CSB-EP812343SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis Alpha-N-acetylgalactosaminidase (nagA) CSB-YP812344SGA
CSB-EP812344SGA
CSB-BP812344SGA
CSB-MP812344SGA
CSB-EP812344SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis Tryptophan synthase beta chain (trpB) CSB-YP812345SGA
CSB-EP812345SGA
CSB-BP812345SGA
CSB-MP812345SGA
CSB-EP812345SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis DNA ligase (ligA), partial CSB-YP812346SGA
CSB-EP812346SGA
CSB-BP812346SGA
CSB-MP812346SGA
CSB-EP812346SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis Maf-like protein SO_2782 (SO_2782) CSB-YP812347SGA
CSB-EP812347SGA
CSB-BP812347SGA
CSB-MP812347SGA
CSB-EP812347SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis Phosphoribosylformylglycinamidine cyclo-ligase (purM) CSB-YP812348SGA
CSB-EP812348SGA
CSB-BP812348SGA
CSB-MP812348SGA
CSB-EP812348SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis Malonyl-CoA O-methyltransferase BioC (bioC) CSB-YP812349SGA
CSB-EP812349SGA
CSB-BP812349SGA
CSB-MP812349SGA
CSB-EP812349SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis Putative phosphotransferase SO_2645 (SO_2645) CSB-YP812350SGA
CSB-EP812350SGA
CSB-BP812350SGA
CSB-MP812350SGA
CSB-EP812350SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產生大量蛋白質、修改基因序列和制造有用的商業(yè)產品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據他們開發(fā)重組蛋白的經驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產,在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據重組蛋白的不同應用,對其生物活性有不同的要求。但是我們如何確保蛋白質的產生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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