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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Staphylococcus epidermidis Protein RecA (recA) CSB-YP813250SLD
CSB-EP813250SLD
CSB-BP813250SLD
CSB-MP813250SLD
CSB-EP813250SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis GTP-sensing transcriptional pleiotropic repressor CodY (codY) CSB-YP813251SLD
CSB-EP813251SLD
CSB-BP813251SLD
CSB-MP813251SLD
CSB-EP813251SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Phosphate acyltransferase (plsX) CSB-YP813252SLD
CSB-EP813252SLD
CSB-BP813252SLD
CSB-MP813252SLD
CSB-EP813252SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Carbamoyl-phosphate synthase small chain (carA) CSB-YP813253SLD
CSB-EP813253SLD
CSB-BP813253SLD
CSB-MP813253SLD
CSB-EP813253SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Ribonuclease HIII (rnhC) CSB-YP813254SLD
CSB-EP813254SLD
CSB-BP813254SLD
CSB-MP813254SLD
CSB-EP813254SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Phosphoribosylformylglycinamidine synthase 2 (purL), partial CSB-YP813255SLD
CSB-EP813255SLD
CSB-BP813255SLD
CSB-MP813255SLD
CSB-EP813255SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Probable quinol oxidase subunit 1 (qoxB), partial CSB-YP813256SLD
CSB-EP813256SLD
CSB-BP813256SLD
CSB-MP813256SLD
CSB-EP813256SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Peptide chain release factor 3 (prfC), partial CSB-YP813257SLD
CSB-EP813257SLD
CSB-BP813257SLD
CSB-MP813257SLD
CSB-EP813257SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Chaperone protein ClpB (clpB), partial CSB-YP813258SLD
CSB-EP813258SLD
CSB-BP813258SLD
CSB-MP813258SLD
CSB-EP813258SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Na (+)/H (+) antiporter subunit C1 (mnhC1), partial CSB-YP813259SLD1
CSB-EP813259SLD1
CSB-BP813259SLD1
CSB-MP813259SLD1
CSB-EP813259SLD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Lipoyl synthase (lipA) CSB-YP813260SLD
CSB-EP813260SLD
CSB-BP813260SLD
CSB-MP813260SLD
CSB-EP813260SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis UvrABC system protein A (uvrA), partial CSB-YP813261SLD
CSB-EP813261SLD
CSB-BP813261SLD
CSB-MP813261SLD
CSB-EP813261SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Iron-sulfur cluster repair protein ScdA (scdA) CSB-YP813262SLD
CSB-EP813262SLD
CSB-BP813262SLD
CSB-MP813262SLD
CSB-EP813262SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Alcohol dehydrogenase (adh) CSB-YP813263SLD
CSB-EP813263SLD
CSB-BP813263SLD
CSB-MP813263SLD
CSB-EP813263SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Probable branched-chain-amino-acid aminotransferase (ilvE) CSB-YP813264SLD
CSB-EP813264SLD
CSB-BP813264SLD
CSB-MP813264SLD
CSB-EP813264SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Transcriptional regulator CtsR (ctsR) CSB-YP813265SLD
CSB-EP813265SLD
CSB-BP813265SLD
CSB-MP813265SLD
CSB-EP813265SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Histidinol dehydrogenase (hisD) CSB-YP813266SLD
CSB-EP813266SLD
CSB-BP813266SLD
CSB-MP813266SLD
CSB-EP813266SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Uncharacterized response regulatory protein SE_0165 (SE_0165) CSB-YP813267SLD
CSB-EP813267SLD
CSB-BP813267SLD
CSB-MP813267SLD
CSB-EP813267SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Transcriptional regulatory protein walR (walR) CSB-YP813268SLD
CSB-EP813268SLD
CSB-BP813268SLD
CSB-MP813268SLD
CSB-EP813268SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis tRNA dimethylallyltransferase (miaA) CSB-YP813269SLD
CSB-EP813269SLD
CSB-BP813269SLD
CSB-MP813269SLD
CSB-EP813269SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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