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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Human Lactosylceramide alpha-2,3-sialyltransferase (ST3GAL5), partial CSB-EP890769HU 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Escherichia coli O6:H1 Cardiolipin synthase A (clsA) , partial CSB-EP005985EGX1 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Bovine Isoamyl acetate-hydrolyzing esterase 1 homolog (IAH1) CSB-EP664861BO 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Mouse Prolyl endopeptidase (Prep) CSB-EP018664MO 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Escherichia coli NADPH-dependent ferric-chelate reductase (yqjH) CSB-EP671608ENV 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Human TAR DNA-binding protein 43 (TARDBP) CSB-EP023129HU(A4)a0 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Drosophila melanogaster 5-hydroxytryptamine receptor 2B (5-HT1B), partial CSB-EP010888DLU1 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Human cytomegalovirus Protein UL141 (UL141), partial CSB-EP757720HWWb1 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Human DNA replication ATP-dependent helicase/nuclease DNA2 (DNA2), partial CSB-EP006982HU 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Mouse Cysteine protease ATG4B (Atg4b) CSB-EP807332MO 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Human Poly [ADP-ribose] polymerase 1 (PARP11), partial CSB-EP017459HUc7 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Mouse Transient receptor potential cation channel subfamily V member 4 (Trpv4), partial CSB-EP875332MO4e1 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Human Sushi, von Willebrand factor type A, EGF and pentraxin domain-containing protein 1 (SVEP1), partial CSB-EP689809HUb1 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Human Adipocyte plasma membrane-associated protein (APMAP), partial CSB-EP884629HUb1 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Mouse Cysteine protease ATG4B (Atg4b) CSB-EP807332MOb1 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Human Cyclic AMP-dependent transcription factor ATF-7 (ATF7), partial CSB-EP002279HUc7 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Rabbit Interleukin-10 (IL10) CSB-EP874667RBc7 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Human Guanine nucleotide-binding protein G (i) subunit alpha-3 (GNAI3) CSB-EP009591HU(A4) 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Human Carboxylesterase 3 (CES3) CSB-EP005260HU 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Human Vigilin (HDLBP), partial CSB-EP010257HU 1mg(1mg*1 or 500ug*2)/100ug/20ug

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產(chǎn)生大量蛋白質、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產(chǎn)生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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