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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Human Protein cereblon (CRBN), partial, Biotinylated CSB-EP842761HU1-B 1mg/100ug/20ug
Recombinant Human Atrial natriuretic peptide-converting enzyme (CORIN), partial CSB-EP896913HU1 1mg/100ug/20ug
Recombinant Severe acute respiratory syndrome coronavirus 2 Spike glycoprotein (S), partial (L452R,T478K) CSB-EP3324GMY14(M1) 1mg/100ug
Recombinant Escherichia coli K88 fimbrial protein AC (faeG) CSB-EP320337ENL 1mg/100ug/20ug
Recombinant Human Protein FAM167B (FAM167B) CSB-EP871572HU 1mg/100ug/20ug
Recombinant Human Apoptosis-associated speck-like protein containing a CARD (PYCARD), partial, Biotinylated CSB-EP890936HU1-B 1mg/100ug/20ug
Recombinant Human Apoptosis-associated speck-like protein containing a CARD(PYCARD), Biotinylated CSB-EP890936HU-B 1mg/100ug/20ug
Recombinant Human Apoptosis-associated speck-like protein containing a CARD (PYCARD), Partial CSB-EP890936HU2 1mg/100ug/20ug
Recombinant Mouse Tripartite motif-containing protein 54 (Trim54) CSB-EP887662MO 1mg/100ug/20ug
Recombinant Helicobacter pylori Protein translocase subunit SecA (secA) , Partial CSB-EP481252HUX 1mg/100ug/20ug
Recombinant Halalkalibacterium halodurans Agmatinase (speB) CSB-EP873007BQS 1mg/100ug/20ug
Recombinant Pig von Willebrand factor (VWF), partial CSB-EP025960PI 1mg/100ug/20ug
Recombinant Human Adenylate cyclase type 3 (ADCY3) , Partial CSB-EP001339HU 1mg/100ug/20ug
Recombinant Rat Aggrecan core protein (Acan), partial CSB-EP001130RA 1mg/100ug/20ug
Recombinant Human Vitamin K-dependent protein C(PROC) CSB-EP018742HU 1mg/100ug/20ug
Recombinant Human Heparan-sulfate 6-O-sulfotransferase 1 (HS6ST1), partial CSB-EP010756HU1 1mg/100ug/20ug
Recombinant Mouse Double-stranded RNA-specific adenosine deaminase (Adar), partial CSB-EP859990MO 1mg/100ug/20ug
Recombinant Escherichia coli Cellulose synthase operon protein C (bcsC) CSB-EP330747ENV(A4) 1mg/100ug/20ug
Recombinant Human parainfluenza 2 virus Nucleoprotein(N) CSB-EP322017HPC(A4) 1mg/100ug/20ug
Recombinant Human Beta-crystallin B3 (CRYBB3) CSB-EP006015HU 1mg/100ug/20ug

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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