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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Human NAD-dependent protein deacetylase sirtuin-1 (SIRT1) CSB-EP822202HUc7 1mg/100ug/20ug
Recombinant Mouse Glycerol-3-phosphate dehydrogenase [NAD (+)], cytoplasmic (Gpd1) CSB-EP009709MO 1mg/100ug/20ug
Recombinant Enterobacteria phage 933W Shiga-like toxin 2 subunit B (stxB2) CSB-EP357663ECCc7 1mg/100ug/20ug
Recombinant Pantoea stewartii subsp. stewartii Transcriptional activator protein esaR (esaR) CSB-EP347483EGK 1mg/100ug/20ug
Recombinant Mouse Cytochrome c oxidase subunit 4 isoform 1, mitochondrial (Cox4i1), partial CSB-EP005832MO1 1mg/100ug/20ug
Recombinant Human Cyclin-dependent kinase inhibitor 1 (CDKN1A) CSB-EP005086HUc0 1mg/100ug/20ug
Recombinant Human Z-DNA-binding protein 1 (ZBP1) CSB-EP861990HUb1 1mg/100ug/20ug
Recombinant Human Ras-related protein Rab-6B (RAB6B) CSB-EP873642HUa0 1mg/100ug/20ug
Recombinant Human Ras-related protein Rab-33A (RAB33A) CSB-EP613485HU 1mg/100ug/20ug
Recombinant Mouse Fatty acid-binding protein, adipocyte (Fabp4) CSB-EP007945MO 1mg/100ug/20ug
Recombinant Vaccinia virus Protein C15/B21 CSB-EP323323VAA 1mg/100ug/20ug
Recombinant Human Interleukin-19 (IL19) CSB-EP883391HU 1mg/100ug/20ug
Recombinant Human Gastric triacylglycerol lipase (LIPF) CSB-EP012976HU 1mg/100ug/20ug
Recombinant Human Apoptosis-associated speck-like protein containing a CARD (PYCARD), Partial CSB-EP890936HU2b0 1mg/100ug/20ug
Recombinant Human Growth/differentiation factor 5 (GDF5) CSB-EP009349HU 1mg/100ug/20ug
Recombinant Mouse Phosphatidylinositol 3-kinase regulatory subunit alpha (Pik3r1) CSB-EP018003MO 1mg/100ug/20ug
Recombinant Human Apoptosis-associated speck-like protein containing a CARD (PYCARD), Partial CSB-EP890936HU2a0 1mg/100ug/20ug
Recombinant Human Cytochrome P450 3A7 (CYP3A7) CSB-EP006444HU 1mg/100ug/20ug
Recombinant Human papillomavirus type 16 Protein E6 (E6) CSB-EP365854HMLc7 1mg/100ug/20ug
Recombinant Mouse Peroxiredoxin-6 (Prdx6) CSB-EP018659MO 1mg/100ug/20ug

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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