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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bos indicus x Bos taurus Kappa-casein, partial (R118C) CSB-EP5324BUM1(M) 1mg/100ug/20ug
Recombinant Bos indicus x Bos taurus Alpha-S1-casein, partial CSB-EP5630BUM(M) 1mg/100ug/20ug
Recombinant Bos indicus x Bos taurus Alpha-S1-casein, partial CSB-EP5630BUM1(M) 1mg/100ug/20ug
Recombinant Mouse Angiopoietin-like protein 8 (Angptl8) CSB-EP844436MOc0 1mg/100ug/20ug
Recombinant Human Glutaminase kidney isoform, mitochondrial (GLS), partial, Biotinylated CSB-EP009528HU(F1)-B 1mg/100ug/20ug
Recombinant Mouse IgG receptor FcRn large subunit p51 (Fcgrt), partial CSB-EP717217MO 1mg/100ug/20ug
Recombinant Shigella flexneri Putative multidrug resistance protein MdtA (mdtA) CSB-EP774494SZB 1mg/100ug/20ug
Recombinant Human Coronin-1A (CORO1A) CSB-EP005813HU 1mg/100ug/20ug
Recombinant Escherichia coli Colicin-E9 immunity protein (imm) CSB-EP318373ENL 1mg/100ug/20ug
Recombinant Escherichia coli Multidrug resistance protein K (emrK), partial CSB-EP345216ENV 1mg/100ug/20ug
Recombinant Shigella flexneri Outer membrane protein assembly factor BamC (bamC) CSB-EP364253SZB 1mg/100ug/20ug
Recombinant Mouse Ubiquitin carboxyl-terminal hydrolase 30 (Usp30), partial CSB-EP668883MO 1mg/100ug/20ug
Recombinant Bartonella quintana 10 kDa chaperonin (groES) CSB-EP760364BSH 1mg/100ug/20ug
Recombinant Human Eukaryotic translation initiation factor 4E-binding protein 1 (EIF4EBP1) CSB-EP623815HU 1mg/100ug/20ug
Recombinant Human E3 ubiquitin-protein ligase XIAP (XIAP) (1-1:M→MM) CSB-EP026193HU(M) 1mg/100ug/20ug
Recombinant Paramacrobiotus richtersi Cytosolic-abundant heat soluble protein 106094 (CAHS?106094) CSB-EP5869GXC 1mg/100ug/20ug
Recombinant Azotobacter vinelandii Uncharacterized protein CSB-EP5894DPY 1mg/100ug/20ug
Recombinant Bacillus cereus Metallo-beta-lactamase type 2 (blm) CSB-EP361221BQJ 1mg/100ug/20ug
Recombinant Human Ribonucleoside-diphosphate reductase large subunit (RRM1) CSB-EP020518HUc7 1mg/100ug/20ug
Recombinant Human YTH domain-containing family protein 2 (YTHDF2), partial CSB-EP896902HU2 1mg/100ug/20ug

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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