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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant African swine fever virus Major capsid protein (Ba71V-081), partial CSB-EP334921AEJ 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Human Deformed epidermal autoregulatory factor 1 homolog (DEAF1) CSB-EP006644HU 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Human herpesvirus 1 Envelope glycoprotein D (gD), partial CSB-EP382935HSP1 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Arabidopsis thaliana 23.6 kDa heat shock protein, mitochondrial (HSP23.6) CSB-EP822140DOA 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Human Methyltransferase-like protein 7B (METTL7B) CSB-EP751014HU 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Xenopus tropicalis Transcription factor Sox-1 (sox1) CSB-EP022417XBF 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Human Zinc finger protein ZIC 4 (ZIC4) CSB-EP850841HU 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Human B-cell CLL/lymphoma 9 protein (BCL9), partial CSB-EP002631HU 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Human Plasma alpha-L-fucosidase (FUCA2) CSB-EP863122HU 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Human respiratory syncytial virus B Matrix M2-1 (M2-1) CSB-EP518618HXK 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Arabidopsis thaliana Defensin-like protein 16 (PDF1.2A) CSB-EP887761DOA 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Human Mucin-21 (MUC21), partial CSB-EP713107HU 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Mouse Solute carrier family 2, facilitated glucose transporter member 1 (Slc2a1), partial CSB-EP021546MO 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Human adenovirus B serotype 3 Hexon protein (L3), partial CSB-EP334192HIG 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Methanosarcina barkeri Pyrrolysine--tRNA ligase (pylS) CSB-EP673888MSM 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Rat Myosin-9 (Myh9), partial CSB-EP717285RA 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Human papillomavirus type 11 Replication protein E1 (E1), partial CSB-EP366087HMG 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Reovirus type 3 Outer capsid protein lambda-2 (L2), partial CSB-EP318819RCH 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant?Rat?NAD-dependent?protein?deacetylase (Sirt3) CSB-EP848095RA 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Escherichia coli O157:H7 Metalloprotease stcE (stcE), partial CSB-EP530572EOD 1mg(1mg*1 or 500ug*2)/100ug/20ug

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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