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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Putative diacyglycerol O-acyltransferase Rv0221/MT0231 (Rv0221, MT0231) CSB-YP309110MVZ
CSB-EP309110MVZ
CSB-BP309110MVZ
CSB-MP309110MVZ
CSB-EP309110MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia Beta-lactamase L2 CSB-YP309111SLU
CSB-EP309111SLU
CSB-BP309111SLU
CSB-MP309111SLU
CSB-EP309111SLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Putative transcriptional regulator yvhJ (yvhJ), partial CSB-YP309112BRJ
CSB-EP309112BRJ
CSB-BP309112BRJ
CSB-MP309112BRJ
CSB-EP309112BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Putative acyl--CoA ligase YdaB (ydaB), partial CSB-YP309113BRJ
CSB-EP309113BRJ
CSB-BP309113BRJ
CSB-MP309113BRJ
CSB-EP309113BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis DNA topoisomerase 3 (topB), partial CSB-YP309114BRJ
CSB-EP309114BRJ
CSB-BP309114BRJ
CSB-MP309114BRJ
CSB-EP309114BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Putative 8-oxo-dGTP diphosphatase (mutT) CSB-YP309115BRJ
CSB-EP309115BRJ
CSB-BP309115BRJ
CSB-MP309115BRJ
CSB-EP309115BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein ydbB (ydbB) CSB-YP309116BRJ
CSB-EP309116BRJ
CSB-BP309116BRJ
CSB-MP309116BRJ
CSB-EP309116BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase (murF) CSB-YP309117BRJ
CSB-EP309117BRJ
CSB-BP309117BRJ
CSB-MP309117BRJ
CSB-EP309117BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein ydcF (ydcF) CSB-YP309118BRJ
CSB-EP309118BRJ
CSB-BP309118BRJ
CSB-MP309118BRJ
CSB-EP309118BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Putative uncharacterized protein ydcP (ydcP) CSB-YP309119BRJ
CSB-EP309119BRJ
CSB-BP309119BRJ
CSB-MP309119BRJ
CSB-EP309119BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein ydcS (ydcS) CSB-YP309120BRJ
CSB-EP309120BRJ
CSB-BP309120BRJ
CSB-MP309120BRJ
CSB-EP309120BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Probable endopeptidase YddH (yddH) CSB-YP309121BRJ
CSB-EP309121BRJ
CSB-BP309121BRJ
CSB-MP309121BRJ
CSB-EP309121BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis UPF0173 protein yddR (yddR) CSB-YP309122BRJ
CSB-EP309122BRJ
CSB-BP309122BRJ
CSB-MP309122BRJ
CSB-EP309122BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized HTH-type transcriptional regulator ydeF (ydeF) CSB-YP309123BRJ
CSB-EP309123BRJ
CSB-BP309123BRJ
CSB-MP309123BRJ
CSB-EP309123BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized HTH-type transcriptional regulator ydeL (ydeL) CSB-YP309124BRJ
CSB-EP309124BRJ
CSB-BP309124BRJ
CSB-MP309124BRJ
CSB-EP309124BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis HTH-type transcriptional repressor AseR (aseR) CSB-YP309125BRJ
CSB-EP309125BRJ
CSB-BP309125BRJ
CSB-MP309125BRJ
CSB-EP309125BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized HTH-type transcriptional regulator ydfD (ydfD) CSB-YP309126BRJ
CSB-EP309126BRJ
CSB-BP309126BRJ
CSB-MP309126BRJ
CSB-EP309126BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Putative oxidoreductase mhqP (mhqP), partial CSB-YP309127BRJ1
CSB-EP309127BRJ1
CSB-BP309127BRJ1
CSB-MP309127BRJ1
CSB-EP309127BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein ydgD (ydgD), partial CSB-YP309128BRJ1
CSB-EP309128BRJ1
CSB-BP309128BRJ1
CSB-MP309128BRJ1
CSB-EP309128BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein ywqB (ywqB), partial CSB-YP309129BRJ
CSB-EP309129BRJ
CSB-BP309129BRJ
CSB-MP309129BRJ
CSB-EP309129BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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