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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Saccharomyces cerevisiae Proteasome component C7-alpha (SCL1) CSB-YP321919SVG
CSB-EP321919SVG
CSB-BP321919SVG
CSB-MP321919SVG
CSB-EP321919SVG-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dickeya dadantii Oligogalacturonate lyase (ogl) CSB-YP321920DKD
CSB-EP321920DKD
CSB-BP321920DKD
CSB-MP321920DKD
CSB-EP321920DKD-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized proline-rich protein CSB-YP321921OCZ
CSB-EP321921OCZ
CSB-BP321921OCZ
CSB-MP321921OCZ
CSB-EP321921OCZ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae CCA tRNA nucleotidyltransferase, mitochondrial (CCA1), partial CSB-YP321922SVG
CSB-EP321922SVG
CSB-BP321922SVG
CSB-MP321922SVG
CSB-EP321922SVG-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Superoxide dismutase [Fe], chloroplastic (SODB), partial CSB-YP321923DOA
CSB-EP321923DOA
CSB-BP321923DOA
CSB-MP321923DOA
CSB-EP321923DOA-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rotavirus A Outer capsid glycoprotein VP7 CSB-YP321924ROG
CSB-EP321924ROG
CSB-BP321924ROG
CSB-MP321924ROG
CSB-EP321924ROG-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Autographa californica nuclear polyhedrosis virus Late expression factor 11 (LEF-11) CSB-YP321925ARA
CSB-EP321925ARA
CSB-BP321925ARA
CSB-MP321925ARA
CSB-EP321925ARA-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Triticum aestivum Gamma-gliadin CSB-YP321926TQN
CSB-EP321926TQN
CSB-BP321926TQN
CSB-MP321926TQN
CSB-EP321926TQN-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caulobacter crescentus Protein flaF (flaF) CSB-YP321927DQO
CSB-EP321927DQO
CSB-BP321927DQO
CSB-MP321927DQO
CSB-EP321927DQO-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Raphanus sativus Late embryogenesis abundant protein, partial CSB-YP321928RJP
CSB-BP321928RJP
CSB-MP321928RJP
CSB-EP321928RJP-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Periodic tryptophan protein 1 (PWP1), partial CSB-YP321929SVG
CSB-EP321929SVG
CSB-BP321929SVG
CSB-MP321929SVG
CSB-EP321929SVG-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi HTH-type transcriptional regulator luxR (luxR) CSB-YP321930VFD
CSB-EP321930VFD
CSB-BP321930VFD
CSB-MP321930VFD
CSB-EP321930VFD-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Retron EC67 DNA adenine methylase CSB-YP321931ENL
CSB-EP321931ENL
CSB-BP321931ENL
CSB-MP321931ENL
CSB-EP321931ENL-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative uncharacterized protein ORFA in retron EC67 CSB-YP321932ENL
CSB-EP321932ENL
CSB-BP321932ENL
CSB-MP321932ENL
CSB-EP321932ENL-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant DinI-like protein in retron EC67 CSB-YP321933ENL
CSB-EP321933ENL
CSB-BP321933ENL
CSB-MP321933ENL
CSB-EP321933ENL-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative uncharacterized protein ORFH in retron EC67 CSB-YP321934ENL
CSB-EP321934ENL
CSB-BP321934ENL
CSB-MP321934ENL
CSB-EP321934ENL-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Retron EC67 protein CSB-YP321935ENL
CSB-EP321935ENL
CSB-BP321935ENL
CSB-MP321935ENL
CSB-EP321935ENL-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila funebris RNA-directed DNA polymerase from mobile element jockey (pol), partial CSB-YP321936DGA
CSB-EP321936DGA
CSB-BP321936DGA
CSB-MP321936DGA
CSB-EP321936DGA-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Nucleic-acid-binding protein from mobile element jockey (gag), partial CSB-YP321937DLU
CSB-EP321937DLU
CSB-BP321937DLU
CSB-MP321937DLU
CSB-EP321937DLU-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Tetracycline repressor protein class E (tetR) CSB-YP321938ENL
CSB-BP321938ENL
CSB-MP321938ENL
CSB-EP321938ENL-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式，可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中，除了表達系統(tǒng)之外，還面臨著一系列令人困惑的選擇，如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時，你必須做出很多決定。如果你選擇的明智，你將獲得高質(zhì)量的重組蛋白，后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定，你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里，來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示，可以幫助你做出明智的決策。

第一條：重組蛋白的生產(chǎn)，在這篇文章中，我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條：如何選擇合適的表達載體?

表達載體是一種DNA分子，它攜帶特定的基因進入宿主細胞，并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達，經(jīng)過多年的發(fā)展，表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示，本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條：如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用，對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題，并提供一些解決方案。除了以上文章，我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外，我們還為我們的客戶提供重組蛋白表達服務(wù)，您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問，涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等，您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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