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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacillus subtilis Flagellar protein fliS (fliS) CSB-YP331069BRJ
CSB-EP331069BRJ
CSB-BP331069BRJ
CSB-MP331069BRJ
CSB-EP331069BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Translocation protein SEC72 (SEC72) CSB-YP331070SVG
CSB-EP331070SVG
CSB-BP331070SVG
CSB-MP331070SVG
CSB-EP331070SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Putative transition state regulator Abh (abh) CSB-YP331071BRJ
CSB-EP331071BRJ
CSB-BP331071BRJ
CSB-MP331071BRJ
CSB-EP331071BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uracil permease (pyrP), partial CSB-YP331072BRJ
CSB-EP331072BRJ
CSB-BP331072BRJ
CSB-MP331072BRJ
CSB-EP331072BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Polyhomeotic-proximal chromatin protein (ph-p), partial CSB-YP331073DLU
CSB-EP331073DLU
CSB-BP331073DLU
CSB-MP331073DLU
CSB-EP331073DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (gpmI), partial CSB-YP331074BRJ
CSB-EP331074BRJ
CSB-BP331074BRJ
CSB-MP331074BRJ
CSB-EP331074BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis GTP-sensing transcriptional pleiotropic repressor CodY (codY) CSB-YP331075BRJ
CSB-EP331075BRJ
CSB-BP331075BRJ
CSB-MP331075BRJ
CSB-EP331075BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Probable endonuclease III (nth) CSB-YP331076BRJ
CSB-EP331076BRJ
CSB-BP331076BRJ
CSB-MP331076BRJ
CSB-EP331076BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Penicillin-binding protein 1A/1B (ponA), partial CSB-YP331077BRJ
CSB-BP331077BRJ
CSB-MP331077BRJ
CSB-EP331077BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Phage-like element PBSX protein xepA (xepA) CSB-YP331078BRJ
CSB-EP331078BRJ
CSB-BP331078BRJ
CSB-MP331078BRJ
CSB-EP331078BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Intracellular proteinase inhibitor (ipi) CSB-YP331079BRJ
CSB-EP331079BRJ
CSB-BP331079BRJ
CSB-MP331079BRJ
CSB-EP331079BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yvyG (yvyG) CSB-YP331080BRJ
CSB-EP331080BRJ
CSB-BP331080BRJ
CSB-MP331080BRJ
CSB-EP331080BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Glutamate synthase [NADPH] large chain (gltA), partial CSB-YP331081BRJ
CSB-EP331081BRJ
CSB-BP331081BRJ
CSB-MP331081BRJ
CSB-EP331081BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Proton glutamate symport protein (gltP), partial CSB-YP331082BRJ
CSB-EP331082BRJ
CSB-BP331082BRJ
CSB-MP331082BRJ
CSB-EP331082BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Beta-lactamase (penP) CSB-YP331083BRJ
CSB-EP331083BRJ
CSB-BP331083BRJ
CSB-MP331083BRJ
CSB-EP331083BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli D-galactarate dehydratase (garD) CSB-YP331084ENV
CSB-EP331084ENV
CSB-BP331084ENV
CSB-MP331084ENV
CSB-EP331084ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli High-affinity gluconate transporter (gntT), partial CSB-YP331085ENV
CSB-EP331085ENV
CSB-BP331085ENV
CSB-MP331085ENV
CSB-EP331085ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Multidrug-efflux transporter 2 regulator (bltR) CSB-YP331086BRJ
CSB-EP331086BRJ
CSB-BP331086BRJ
CSB-MP331086BRJ
CSB-EP331086BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Beta-N-acetylglucosaminidase (lytD), partial CSB-YP331087BRJ
CSB-EP331087BRJ
CSB-BP331087BRJ
CSB-MP331087BRJ
CSB-EP331087BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein CapE (capE), partial CSB-YP331088FKZ
CSB-EP331088FKZ
CSB-BP331088FKZ
CSB-MP331088FKZ
CSB-EP331088FKZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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