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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacillus subtilis Ribosomal RNA small subunit methyltransferase A (rsmA) CSB-YP336297BRJ
CSB-EP336297BRJ
CSB-BP336297BRJ
CSB-MP336297BRJ
CSB-EP336297BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Transcription-repair-coupling factor (mfd), partial CSB-YP336298BRJ
CSB-EP336298BRJ
CSB-BP336298BRJ
CSB-MP336298BRJ
CSB-EP336298BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yycE (yycE) CSB-YP336299BRJ
CSB-EP336299BRJ
CSB-BP336299BRJ
CSB-MP336299BRJ
CSB-EP336299BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yybT (yybT), partial CSB-YP336300BRJ
CSB-EP336300BRJ
CSB-BP336300BRJ
CSB-MP336300BRJ
CSB-EP336300BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized lipoprotein yybP (yybP) CSB-YP336301BRJ
CSB-EP336301BRJ
CSB-BP336301BRJ
CSB-MP336301BRJ
CSB-EP336301BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized ABC transporter ATP-binding protein YybJ (yybJ) CSB-YP336302BRJ
CSB-EP336302BRJ
CSB-BP336302BRJ
CSB-MP336302BRJ
CSB-EP336302BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized MFS-type transporter yybF (yybF), partial CSB-YP336303BRJ
CSB-EP336303BRJ
CSB-BP336303BRJ
CSB-MP336303BRJ
CSB-EP336303BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized HTH-type transcriptional regulator yybA (yybA) CSB-YP336304BRJ
CSB-EP336304BRJ
CSB-BP336304BRJ
CSB-MP336304BRJ
CSB-EP336304BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yyaP (yyaP), partial CSB-YP336305BRJ1
CSB-EP336305BRJ1
CSB-BP336305BRJ1
CSB-MP336305BRJ1
CSB-EP336305BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized transporter yyaM (yyaM), partial CSB-YP336306BRJ
CSB-EP336306BRJ
CSB-BP336306BRJ
CSB-MP336306BRJ
CSB-EP336306BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Catabolite control protein B (ccpB) CSB-YP336307BRJ
CSB-EP336307BRJ
CSB-BP336307BRJ
CSB-MP336307BRJ
CSB-EP336307BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yyaC (yyaC) CSB-YP336308BRJ
CSB-EP336308BRJ
CSB-BP336308BRJ
CSB-MP336308BRJ
CSB-EP336308BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yaaC (yaaC) CSB-YP336309BRJ
CSB-EP336309BRJ
CSB-BP336309BRJ
CSB-MP336309BRJ
CSB-EP336309BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Spore germination protein yaaH (yaaH) CSB-YP336310BRJ
CSB-EP336310BRJ
CSB-BP336310BRJ
CSB-MP336310BRJ
CSB-EP336310BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yaaO (yaaO) CSB-YP336311BRJ
CSB-EP336311BRJ
CSB-BP336311BRJ
CSB-MP336311BRJ
CSB-EP336311BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Stage 0 sporulation protein yaaT (yaaT) CSB-YP336312BRJ
CSB-EP336312BRJ
CSB-BP336312BRJ
CSB-MP336312BRJ
CSB-EP336312BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Ribonuclease M5 (rnmV) CSB-YP336313BRJ
CSB-EP336313BRJ
CSB-BP336313BRJ
CSB-MP336313BRJ
CSB-EP336313BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yabK (yabK) CSB-YP336314BRJ
CSB-EP336314BRJ
CSB-BP336314BRJ
CSB-MP336314BRJ
CSB-EP336314BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Spore protein yabP (yabP) CSB-YP336315BRJ
CSB-EP336315BRJ
CSB-BP336315BRJ
CSB-MP336315BRJ
CSB-EP336315BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis tRNA (Ile)-lysidine synthase CSB-YP336316BRJ
CSB-EP336316BRJ
CSB-BP336316BRJ
CSB-MP336316BRJ
CSB-EP336316BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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