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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Human Cell surface glycoprotein MUC18 (MCAM), partial CSB-RP104394h 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Human NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 (NDUFA2), partial CSB-RP029854h 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Human CD40 ligand (CD40LG), partial CSB-EP004937HU4 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Human Rho guanine nucleotide exchange factor 7 (ARHGEF7), partial CSB-RP014454h(C) 1mg(1mg*1 or 500ug*2)/500μg/200μg/100μg/50μg/10μg
Recombinant Mouse Tryptase (Tpsab1), partial CSB-YP024127MO 1mg/100ug/20ug
Recombinant Mouse CD81 antigen (Cd81), partial CSB-EP004960MO 1mg(1mg*1 or 500ug*2)/100μg/20μg
Recombinant Human Eukaryotic translation initiation factor 4E-binding protein 2 (EIF4EBP2) CSB-EP007563HU 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Human Eukaryotic translation initiation factor 4E-binding protein 2 (EIF4EBP2) CSB-YP007563HU 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Human PHD finger-like domain-containing protein 5A (PHF5A) CSB-EP017915HU 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Human PRKCA-binding protein (PICK1), partial CSB-RP054454h 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Human AP-1 complex subunit sigma-3 (AP1S3) CSB-EP001868HU 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Human Leucine-rich repeat flightless-interacting protein 1 (LRRFIP1), partial CSB-EP013175HU 1mg(1mg*1 or 500ug*2)/100μg/20μg
Recombinant Human Striated muscle preferentially expressed protein kinase (SPEG), partial CSB-EP022529HU 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Human Histone H2A.J (H2AFJ) CSB-EP010094HU 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Human MORF4 family-associated protein 1-like 1 (MRFAP1L1) CSB-EP014790HU 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Human DTW domain-containing protein 1 (DTWD1), partial CSB-EP007219HU 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Human TRAF family member-associated NF-kappa-B activator (TANK), partial CSB-EP023116HU 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Human TRAF family member-associated NF-kappa-B activator (TANK), partial CSB-YP023116HU 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Human Follicle-stimulating hormone receptor (FSHR), partial CSB-EP009021HU 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Human RING-box protein 2 (RNF7) CSB-EP019897HU 1mg(1mg*1 or 500ug*2)/100ug/20ug

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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