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重組蛋白

產品名稱 貨號 規(guī)格
Recombinant Bacillus subtilis S-adenosylmethionine synthase (metK) CSB-YP346166BRJ
CSB-EP346166BRJ
CSB-BP346166BRJ
CSB-MP346166BRJ
CSB-EP346166BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Probable beta-lactamase ybxI (ybxI) CSB-YP346167BRJ
CSB-EP346167BRJ
CSB-BP346167BRJ
CSB-MP346167BRJ
CSB-EP346167BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis UPF0721 transmembrane protein yrkJ (yrkJ), partial CSB-YP346168BRJ
CSB-EP346168BRJ
CSB-BP346168BRJ
CSB-MP346168BRJ
CSB-EP346168BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Protein psiE homolog (psiE), partial CSB-YP346169BRJ1
CSB-EP346169BRJ1
CSB-BP346169BRJ1
CSB-MP346169BRJ1
CSB-EP346169BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yqeD (yqeD), partial CSB-YP346170BRJ1
CSB-EP346170BRJ1
CSB-BP346170BRJ1
CSB-MP346170BRJ1
CSB-EP346170BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yqeN (yqeN) CSB-YP346171BRJ
CSB-EP346171BRJ
CSB-BP346171BRJ
CSB-MP346171BRJ
CSB-EP346171BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yqfB (yqfB), partial CSB-YP346172BRJ
CSB-EP346172BRJ
CSB-BP346172BRJ
CSB-MP346172BRJ
CSB-EP346172BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Putative phosphotransferase yqfL (yqfL) CSB-YP346173BRJ
CSB-EP346173BRJ
CSB-BP346173BRJ
CSB-MP346173BRJ
CSB-EP346173BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yqfX (yqfX), partial CSB-YP346174BRJ1
CSB-EP346174BRJ1
CSB-BP346174BRJ1
CSB-MP346174BRJ1
CSB-EP346174BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yqgN (yqgN) CSB-YP346175BRJ
CSB-EP346175BRJ
CSB-BP346175BRJ
CSB-MP346175BRJ
CSB-EP346175BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Lipoteichoic acid synthase-like yqgS (yqgS), partial CSB-YP346176BRJ1
CSB-EP346176BRJ1
CSB-BP346176BRJ1
CSB-MP346176BRJ1
CSB-EP346176BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yqgX (yqgX) CSB-YP346177BRJ
CSB-EP346177BRJ
CSB-BP346177BRJ
CSB-MP346177BRJ
CSB-EP346177BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Transcriptional regulator MntR (mntR) CSB-YP346178BRJ
CSB-EP346178BRJ
CSB-BP346178BRJ
CSB-MP346178BRJ
CSB-EP346178BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis 3-dehydroquinate dehydratase (yqhS) CSB-YP346179BRJ
CSB-EP346179BRJ
CSB-BP346179BRJ
CSB-MP346179BRJ
CSB-EP346179BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Probable phosphate butyryltransferase (yqiS) CSB-YP346180BRJ
CSB-EP346180BRJ
CSB-BP346180BRJ
CSB-MP346180BRJ
CSB-EP346180BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yqjC (yqjC) CSB-YP346181BRJ
CSB-EP346181BRJ
CSB-BP346181BRJ
CSB-MP346181BRJ
CSB-EP346181BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis NADPH dehydrogenase (namA) CSB-YP346182BRJ
CSB-EP346182BRJ
CSB-BP346182BRJ
CSB-MP346182BRJ
CSB-EP346182BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Pantothenate kinase (coaA) CSB-YP346183BRJ
CSB-EP346183BRJ
CSB-BP346183BRJ
CSB-MP346183BRJ
CSB-EP346183BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis UPF0157 protein YqkA (yqkA) CSB-YP346184BRJ
CSB-EP346184BRJ
CSB-BP346184BRJ
CSB-MP346184BRJ
CSB-EP346184BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yqkE (yqkE) CSB-YP346185BRJ
CSB-EP346185BRJ
CSB-BP346185BRJ
CSB-MP346185BRJ
CSB-EP346185BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產生大量蛋白質、修改基因序列和制造有用的商業(yè)產品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產,在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們如何確保蛋白質的產生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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