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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Drosophila melanogaster 39 kDa FK506-binding nuclear protein (FK506-bp1) CSB-YP346941DLU
CSB-EP346941DLU
CSB-BP346941DLU
CSB-MP346941DLU
CSB-EP346941DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter jejuni subsp. jejuni serotype O:2 ATP-dependent Clp protease proteolytic subunit (clpP) CSB-YP346942CZA
CSB-EP346942CZA
CSB-BP346942CZA
CSB-MP346942CZA
CSB-EP346942CZA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis UPF0033 protein yrkF (yrkF) CSB-YP346943BRJ
CSB-EP346943BRJ
CSB-BP346943BRJ
CSB-MP346943BRJ
CSB-EP346943BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis UPF0033 protein yrkI (yrkI) CSB-YP346944BRJ
CSB-EP346944BRJ
CSB-BP346944BRJ
CSB-MP346944BRJ
CSB-EP346944BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized transcriptional regulatory protein yrkP (yrkP) CSB-YP346945BRJ
CSB-EP346945BRJ
CSB-BP346945BRJ
CSB-MP346945BRJ
CSB-EP346945BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized lipoprotein yqeF (yqeF) CSB-YP346946BRJ
CSB-EP346946BRJ
CSB-BP346946BRJ
CSB-MP346946BRJ
CSB-EP346946BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Ribosomal protein L11 methyltransferase (prmA) CSB-YP346947BRJ
CSB-EP346947BRJ
CSB-BP346947BRJ
CSB-MP346947BRJ
CSB-EP346947BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yqeY (yqeY) CSB-YP346948BRJ
CSB-EP346948BRJ
CSB-BP346948BRJ
CSB-MP346948BRJ
CSB-EP346948BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis tRNA (adenine (22)-N (1))-methyltransferase CSB-YP346949BRJ
CSB-EP346949BRJ
CSB-BP346949BRJ
CSB-MP346949BRJ
CSB-EP346949BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Zinc-specific metallo-regulatory protein (zur) CSB-YP346950BRJ
CSB-EP346950BRJ
CSB-BP346950BRJ
CSB-MP346950BRJ
CSB-EP346950BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yqgB (yqgB), partial CSB-YP346951BRJ1
CSB-EP346951BRJ1
CSB-BP346951BRJ1
CSB-MP346951BRJ1
CSB-EP346951BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yqgQ (yqgQ) CSB-YP346952BRJ
CSB-EP346952BRJ
CSB-BP346952BRJ
CSB-MP346952BRJ
CSB-EP346952BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Regulatory protein MgsR (mgsR) CSB-YP346953BRJ
CSB-EP346953BRJ
CSB-BP346953BRJ
CSB-MP346953BRJ
CSB-EP346953BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized ATP-dependent helicase yqhH (yqhH), partial CSB-YP346954BRJ
CSB-EP346954BRJ
CSB-BP346954BRJ
CSB-MP346954BRJ
CSB-EP346954BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis N utilization substance protein B homolog (nusB) CSB-YP346955BRJ
CSB-EP346955BRJ
CSB-BP346955BRJ
CSB-MP346955BRJ
CSB-EP346955BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Leucine dehydrogenase (yqiT) CSB-YP346956BRJ
CSB-EP346956BRJ
CSB-BP346956BRJ
CSB-MP346956BRJ
CSB-EP346956BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Membrane protein insertase MisCB (misCB), partial CSB-YP346957BRJ1
CSB-EP346957BRJ1
CSB-BP346957BRJ1
CSB-MP346957BRJ1
CSB-EP346957BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yqjL (yqjL) CSB-YP346958BRJ
CSB-EP346958BRJ
CSB-BP346958BRJ
CSB-MP346958BRJ
CSB-EP346958BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized oxidoreductase yqjQ (yqjQ) CSB-YP346959BRJ
CSB-EP346959BRJ
CSB-BP346959BRJ
CSB-MP346959BRJ
CSB-EP346959BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yqjU (yqjU), partial CSB-YP346960BRJ
CSB-EP346960BRJ
CSB-BP346960BRJ
CSB-MP346960BRJ
CSB-EP346960BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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