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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Streptomyces rochei Alpha-amylase inhibitor Z-2685 CSB-YP362151SPL
CSB-EP362151SPL
CSB-BP362151SPL
CSB-MP362151SPL
CSB-EP362151SPL-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis Phosphocarrier protein HPr (ptsH) CSB-YP362152ELW
CSB-EP362152ELW
CSB-BP362152ELW
CSB-MP362152ELW
CSB-EP362152ELW-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus Subtilisin (apr) CSB-YP362153BRC
CSB-EP362153BRC
CSB-BP362153BRC
CSB-MP362153BRC
CSB-EP362153BRC-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Columba livia Feather keratin B-4 CSB-YP362154DVK
CSB-EP362154DVK
CSB-BP362154DVK
CSB-MP362154DVK
CSB-EP362154DVK-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ophiophagus hannah Long neurotoxin 3 CSB-YP362155OEL
CSB-EP362155OEL
CSB-BP362155OEL
CSB-MP362155OEL
CSB-EP362155OEL-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus halodurans Endo-1,4-beta-xylanase A (xynA) CSB-YP362156BQS
CSB-EP362156BQS
CSB-BP362156BQS
CSB-MP362156BQS
CSB-EP362156BQS-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus phage PZA Head fiber protein (8.5) CSB-YP362157BBE
CSB-EP362157BBE
CSB-BP362157BBE
CSB-MP362157BBE
CSB-EP362157BBE-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus phage PZA Tail protein (9), partial CSB-YP362158BBE
CSB-EP362158BBE
CSB-BP362158BBE
CSB-MP362158BBE
CSB-EP362158BBE-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus phage PZA Pre-neck appendage protein (12), partial CSB-YP362159BBE
CSB-EP362159BBE
CSB-BP362159BBE
CSB-MP362159BBE
CSB-EP362159BBE-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus phage PZA Lysis protein (14) CSB-YP362160BBE
CSB-EP362160BBE
CSB-BP362160BBE
CSB-MP362160BBE
CSB-EP362160BBE-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus phage PZA Encapsidation protein (16) CSB-YP362161BBE
CSB-EP362161BBE
CSB-BP362161BBE
CSB-MP362161BBE
CSB-EP362161BBE-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Agrobacterium tumefaciens Regulatory protein virG (virG) CSB-YP362162AYS
CSB-EP362162AYS
CSB-BP362162AYS
CSB-MP362162AYS
CSB-EP362162AYS-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Homeotic protein deformed (Dfd), partial CSB-YP362163DLU
CSB-EP362163DLU
CSB-BP362163DLU
CSB-MP362163DLU
CSB-EP362163DLU-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Ras-related protein SEC4 (SEC4) CSB-YP362164SVG
CSB-EP362164SVG
CSB-BP362164SVG
CSB-MP362164SVG
CSB-EP362164SVG-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Suid herpesvirus 1 Envelope glycoprotein G (gG), partial CSB-YP362165SRK1
CSB-EP362165SRK1
CSB-BP362165SRK1
CSB-MP362165SRK1
CSB-EP362165SRK1-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rubella virus Structural polyprotein, partial CSB-YP362166RHS1
CSB-EP362166RHS1
CSB-BP362166RHS1
CSB-MP362166RHS1
CSB-EP362166RHS1-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mason-Pfizer monkey virus Protease (pro) CSB-YP362167MCJ
CSB-EP362167MCJ
CSB-BP362167MCJ
CSB-MP362167MCJ
CSB-EP362167MCJ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mason-Pfizer monkey virus Envelope glycoprotein (env), partial CSB-YP362168MCJ
CSB-EP362168MCJ
CSB-BP362168MCJ
CSB-MP362168MCJ
CSB-EP362168MCJ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas phage phi6 Major outer capsid protein (P8) CSB-YP362169PUV
CSB-EP362169PUV
CSB-BP362169PUV
CSB-MP362169PUV
CSB-EP362169PUV-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas phage phi6 Major envelope protein (P9), partial CSB-YP362170PUV1
CSB-EP362170PUV1
CSB-BP362170PUV1
CSB-MP362170PUV1
CSB-EP362170PUV1-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式，可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中，除了表達(dá)系統(tǒng)之外，還面臨著一系列令人困惑的選擇，如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí)，你必須做出很多決定。如果你選擇的明智，你將獲得高質(zhì)量的重組蛋白，后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定，你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里，來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示，可以幫助你做出明智的決策。

第一條：重組蛋白的生產(chǎn)，在這篇文章中，我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條：如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子，它攜帶特定的基因進(jìn)入宿主細(xì)胞，并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá)，經(jīng)過多年的發(fā)展，表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示，本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條：如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用，對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題，并提供一些解決方案。除了以上文章，我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外，我們還為我們的客戶提供重組蛋白表達(dá)服務(wù)，您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問，涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等，您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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