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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Human WD repeat domain phosphoinositide-interacting protein 4 (WDR45) CSB-EP852773HU 1mg(1mg*1 or 500ug*2)/100μg/20μg
Recombinant Human Sodium/potassium-transporting ATPase subunit gamma (FXYD2) CSB-EP009090HU 1mg(1mg*1 or 500ug*2)/100μg/20μg
Recombinant Human Cofilin-2 (CFL2) CSB-EP005281HU 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Human Mitochondrial import inner membrane translocase subunit TIM14 (DNAJC19) CSB-EP853400HU 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Human Mitochondrial import inner membrane translocase subunit Tim17-B (TIMM17B) CSB-EP023551HU 1mg(1mg*1 or 500ug*2)/100μg/20μg
Recombinant Human Myosin regulatory light chain 2, atrial isoform (MYL7) CSB-EP889545HU 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Human Mitochondrial import inner membrane translocase subunit Tim23 (TIMM23) CSB-EP023553HU 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Burkholderia thailandensis Type III secretion system needle protein (yscF) CSB-YP2103BNU 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Plasmodium falciparum Apical membrane antigen 1 (AMA-1), partial CSB-EP338457PLT 1mg(1mg*1 or 500ug*2)/100μg/20μg
Recombinant Human Breast cancer type 1 susceptibility protein (BRCA1), partial CSB-RP096594h(N) 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Human Histone H2AX (H2AFX), partial CSB-EP010097HU1 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Human TAR DNA-binding protein 43 (TARDBP), partial CSB-YP023129HU 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Human Angiotensinogen (AGT), partial CSB-EP001463HU1 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Bovine Beta-1,4-galactosyltransferase 1 (B4GALT1), partial CSB-EP002513BO 1mg(1mg*1 or 500ug*2)/100μg/20μg
Recombinant Human Butyrophilin subfamily 3 member A1 (BTN3A1), partial CSB-EP002873HU 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Human Desmoglein-3 (DSG3), partial CSB-EP007205HU(A5) 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Human Fms-related tyrosine kinase 3 ligand (FLT3LG), partial CSB-EP008734HU1 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Human Gamma-aminobutyric acid receptor subunit alpha-4 (GABRA4), partial CSB-EP009143HU 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Human Heterogeneous nuclear ribonucleoprotein L (HNRNPL) CSB-EP010612HU(F1) 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Human NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial (NDUFB5), partial CSB-EP015652HU 1mg(1mg*1 or 500ug*2)/100ug/20ug

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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