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重組蛋白

產品名稱 貨號 規(guī)格
Recombinant Francisella tularensis subsp. novicida 30S ribosomal protein S4 (rpsD) CSB-YP370865FDW
CSB-EP370865FDW
CSB-BP370865FDW
CSB-MP370865FDW
CSB-EP370865FDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. novicida 50S ribosomal protein L33 (rpmG) CSB-YP370866FDW
CSB-EP370866FDW
CSB-BP370866FDW
CSB-MP370866FDW
CSB-EP370866FDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. novicida Protein TolB (tolB) CSB-YP370867FDW
CSB-EP370867FDW
CSB-BP370867FDW
CSB-MP370867FDW
CSB-EP370867FDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. novicida Glycerol-3-phosphate dehydrogenase [NAD (P)+] CSB-YP370868FDW
CSB-EP370868FDW
CSB-BP370868FDW
CSB-MP370868FDW
CSB-EP370868FDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. novicida Ribosomal RNA large subunit methyltransferase E (rlmE) CSB-YP370869FDW
CSB-EP370869FDW
CSB-BP370869FDW
CSB-MP370869FDW
CSB-EP370869FDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. novicida Isoleucine--tRNA ligase (ileS), partial CSB-YP370870FDW
CSB-EP370870FDW
CSB-BP370870FDW
CSB-MP370870FDW
CSB-EP370870FDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. novicida Ubiquinone/menaquinone biosynthesis methyltransferase ubiE (ubiE) CSB-YP370871FDW
CSB-EP370871FDW
CSB-BP370871FDW
CSB-MP370871FDW
CSB-EP370871FDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. novicida Bifunctional protein GlmU (glmU) CSB-YP370872FDW
CSB-EP370872FDW
CSB-BP370872FDW
CSB-MP370872FDW
CSB-EP370872FDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. novicida Probable glycine dehydrogenase [decarboxylating] subunit 2 (gcvPB) CSB-YP370873FDW
CSB-EP370873FDW
CSB-BP370873FDW
CSB-MP370873FDW
CSB-EP370873FDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. novicida UDP-N-acetylmuramoylalanine--D-glutamate ligase (murD) CSB-YP370874FDW
CSB-EP370874FDW
CSB-BP370874FDW
CSB-MP370874FDW
CSB-EP370874FDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. novicida LPS-assembly protein lptD (lptD), partial CSB-YP370875FDW
CSB-EP370875FDW
CSB-BP370875FDW
CSB-MP370875FDW
CSB-EP370875FDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. novicida Pyridoxal biosynthesis lyase pdxS (pdxS) CSB-YP370876FDW
CSB-EP370876FDW
CSB-BP370876FDW
CSB-MP370876FDW
CSB-EP370876FDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. novicida Ribosomal RNA small subunit methyltransferase H (rsmH) CSB-YP370877FDW
CSB-EP370877FDW
CSB-BP370877FDW
CSB-MP370877FDW
CSB-EP370877FDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. novicida 23S rRNA (uracil (1939)-C (5))-methyltransferase RlmD CSB-YP370878FDW
CSB-EP370878FDW
CSB-BP370878FDW
CSB-MP370878FDW
CSB-EP370878FDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. novicida Serine--tRNA ligase (serS) CSB-YP370879FDW
CSB-EP370879FDW
CSB-BP370879FDW
CSB-MP370879FDW
CSB-EP370879FDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. novicida Protein translocase subunit SecA (secA), partial CSB-YP370880FDW
CSB-EP370880FDW
CSB-BP370880FDW
CSB-MP370880FDW
CSB-EP370880FDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. novicida 50S ribosomal protein L21 (rplU) CSB-YP370881FDW
CSB-EP370881FDW
CSB-BP370881FDW
CSB-MP370881FDW
CSB-EP370881FDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. novicida 50S ribosomal protein L27 (rpmA) CSB-YP370882FDW
CSB-EP370882FDW
CSB-BP370882FDW
CSB-MP370882FDW
CSB-EP370882FDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. novicida Alanine racemase (alr) CSB-YP370883FDW
CSB-EP370883FDW
CSB-BP370883FDW
CSB-MP370883FDW
CSB-EP370883FDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. novicida UPF0176 protein FTN_0789 (FTN_0789) CSB-YP370884FDW
CSB-EP370884FDW
CSB-BP370884FDW
CSB-MP370884FDW
CSB-EP370884FDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產生大量蛋白質、修改基因序列和制造有用的商業(yè)產品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產,在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們如何確保蛋白質的產生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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