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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bacillus thuringiensis 3-isopropylmalate dehydratase large subunit (leuC) CSB-YP371185BOM
CSB-EP371185BOM
CSB-BP371185BOM
CSB-MP371185BOM
CSB-EP371185BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis ATP phosphoribosyltransferase regulatory subunit (hisZ) CSB-YP371186BOM
CSB-EP371186BOM
CSB-BP371186BOM
CSB-MP371186BOM
CSB-EP371186BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis Imidazole glycerol phosphate synthase subunit HisH (hisH) CSB-YP371187BOM
CSB-EP371187BOM
CSB-BP371187BOM
CSB-MP371187BOM
CSB-EP371187BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis Phosphoribosyl-ATP pyrophosphatase (hisE) CSB-YP371188BOM
CSB-EP371188BOM
CSB-BP371188BOM
CSB-MP371188BOM
CSB-EP371188BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis Thiol-disulfide oxidoreductase resA (resA), partial CSB-YP371189BOM1
CSB-EP371189BOM1
CSB-BP371189BOM1
CSB-MP371189BOM1
CSB-EP371189BOM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis Glycerol-3-phosphate dehydrogenase [NAD (P)+] CSB-YP371190BOM
CSB-EP371190BOM
CSB-BP371190BOM
CSB-MP371190BOM
CSB-EP371190BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis Chorismate synthase 1 (aroC1) CSB-YP371191BOM
CSB-EP371191BOM
CSB-BP371191BOM
CSB-MP371191BOM
CSB-EP371191BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis Methylglyoxal synthase (mgsA) CSB-YP371192BOM
CSB-EP371192BOM
CSB-BP371192BOM
CSB-MP371192BOM
CSB-EP371192BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis Pantothenate synthetase (panC) CSB-YP371193BOM
CSB-EP371193BOM
CSB-BP371193BOM
CSB-MP371193BOM
CSB-EP371193BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis Xanthine phosphoribosyltransferase (xpt) CSB-YP371194BOM
CSB-EP371194BOM
CSB-BP371194BOM
CSB-MP371194BOM
CSB-EP371194BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis Aspartate--ammonia ligase (asnA) CSB-YP371195BOM
CSB-EP371195BOM
CSB-BP371195BOM
CSB-MP371195BOM
CSB-EP371195BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis UPF0176 protein BALH_1658 (BALH_1658) CSB-YP371196BOM
CSB-EP371196BOM
CSB-BP371196BOM
CSB-MP371196BOM
CSB-EP371196BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis Homoserine kinase (thrB) CSB-YP371197BOM
CSB-EP371197BOM
CSB-BP371197BOM
CSB-MP371197BOM
CSB-EP371197BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis NH (3)-dependent NAD (+) synthetase CSB-YP371198BOM
CSB-EP371198BOM
CSB-BP371198BOM
CSB-MP371198BOM
CSB-EP371198BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis Metallothiol transferase fosB (fosB) CSB-YP371199BOM
CSB-EP371199BOM
CSB-BP371199BOM
CSB-MP371199BOM
CSB-EP371199BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis 4-hydroxy-2-oxovalerate aldolase (BALH_1844) CSB-YP371200BOM
CSB-EP371200BOM
CSB-BP371200BOM
CSB-MP371200BOM
CSB-EP371200BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis Sec-independent protein translocase protein TatA (tatA), partial CSB-YP371201BOM
CSB-EP371201BOM
CSB-BP371201BOM
CSB-MP371201BOM
CSB-EP371201BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis 5-dehydro-2-deoxygluconokinase (iolC) CSB-YP371202BOM
CSB-EP371202BOM
CSB-BP371202BOM
CSB-MP371202BOM
CSB-EP371202BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis Inosose dehydratase (iolE) CSB-YP371203BOM
CSB-EP371203BOM
CSB-BP371203BOM
CSB-MP371203BOM
CSB-EP371203BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis UPF0421 protein BALH_2468 (BALH_2468), partial CSB-YP371204BOM1
CSB-EP371204BOM1
CSB-BP371204BOM1
CSB-MP371204BOM1
CSB-EP371204BOM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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