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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Shewanella amazonensis ATP-dependent RNA helicase rhlB (rhlB) CSB-YP377768STK
CSB-EP377768STK
CSB-BP377768STK
CSB-MP377768STK
CSB-EP377768STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Diaminopimelate epimerase (dapF) CSB-YP377769STK
CSB-EP377769STK
CSB-BP377769STK
CSB-MP377769STK
CSB-EP377769STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Dihydroxy-acid dehydratase (ilvD), partial CSB-YP377770STK
CSB-EP377770STK
CSB-BP377770STK
CSB-MP377770STK
CSB-EP377770STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Cell division protein ZapB (zapB) CSB-YP377771STK
CSB-EP377771STK
CSB-BP377771STK
CSB-MP377771STK
CSB-EP377771STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis FMN-dependent NADH-azoreductase (azoR) CSB-YP377772STK
CSB-EP377772STK
CSB-BP377772STK
CSB-MP377772STK
CSB-EP377772STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis UPF0345 protein Sama_3424 (Sama_3424) CSB-YP377773STK
CSB-EP377773STK
CSB-BP377773STK
CSB-MP377773STK
CSB-EP377773STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Fe/S biogenesis protein NfuA (nfuA) CSB-YP377774STK
CSB-EP377774STK
CSB-BP377774STK
CSB-MP377774STK
CSB-EP377774STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Ribosomal RNA small subunit methyltransferase J (rsmJ) CSB-YP377775STK
CSB-EP377775STK
CSB-BP377775STK
CSB-MP377775STK
CSB-EP377775STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Bifunctional protein GlmU (glmU) CSB-YP377776STK
CSB-EP377776STK
CSB-BP377776STK
CSB-MP377776STK
CSB-EP377776STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis ATP synthase gamma chain (atpG) CSB-YP377777STK
CSB-EP377777STK
CSB-BP377777STK
CSB-MP377777STK
CSB-EP377777STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis ATP synthase subunit c (atpE), partial CSB-YP377778STK1
CSB-EP377778STK1
CSB-BP377778STK1
CSB-MP377778STK1
CSB-EP377778STK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG), partial CSB-YP377779STK
CSB-EP377779STK
CSB-BP377779STK
CSB-MP377779STK
CSB-EP377779STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nocardioides sp. UPF0133 protein Noca_0318 (Noca_0318) CSB-YP377780NFC
CSB-EP377780NFC
CSB-BP377780NFC
CSB-MP377780NFC
CSB-EP377780NFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nocardioides sp. Pantothenate synthetase (panC) CSB-YP377781NFC
CSB-EP377781NFC
CSB-BP377781NFC
CSB-MP377781NFC
CSB-EP377781NFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nocardioides sp. Aspartate 1-decarboxylase (panD) CSB-YP377782NFC
CSB-EP377782NFC
CSB-BP377782NFC
CSB-MP377782NFC
CSB-EP377782NFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nocardioides sp. Glutamate-1-semialdehyde 2,1-aminomutase (hemL) CSB-YP377783NFC
CSB-EP377783NFC
CSB-BP377783NFC
CSB-MP377783NFC
CSB-EP377783NFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nocardioides sp. NADH-quinone oxidoreductase subunit C (nuoC) CSB-YP377784NFC
CSB-EP377784NFC
CSB-BP377784NFC
CSB-MP377784NFC
CSB-EP377784NFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nocardioides sp. NADH-quinone oxidoreductase subunit N (nuoN), partial CSB-YP377785NFC
CSB-EP377785NFC
CSB-BP377785NFC
CSB-MP377785NFC
CSB-EP377785NFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nocardioides sp. 50S ribosomal protein L33 (rpmG) CSB-YP377786NFC
CSB-EP377786NFC
CSB-BP377786NFC
CSB-MP377786NFC
CSB-EP377786NFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nocardioides sp. 50S ribosomal protein L11 (rplK) CSB-YP377787NFC
CSB-EP377787NFC
CSB-BP377787NFC
CSB-MP377787NFC
CSB-EP377787NFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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