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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Burkholderia mallei Glutamate--tRNA ligase (gltX) CSB-YP379229BPR
CSB-EP379229BPR
CSB-BP379229BPR
CSB-MP379229BPR
CSB-EP379229BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha (accA) CSB-YP379230BPR
CSB-EP379230BPR
CSB-BP379230BPR
CSB-MP379230BPR
CSB-EP379230BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Enolase (eno) CSB-YP379231BPR
CSB-EP379231BPR
CSB-BP379231BPR
CSB-MP379231BPR
CSB-EP379231BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Chaperone protein hscA homolog (hscA), partial CSB-YP379232BPR
CSB-EP379232BPR
CSB-BP379232BPR
CSB-MP379232BPR
CSB-EP379232BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Aspartate 1-decarboxylase (panD) CSB-YP379233BPR
CSB-EP379233BPR
CSB-BP379233BPR
CSB-MP379233BPR
CSB-EP379233BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei UvrABC system protein C (uvrC), partial CSB-YP379234BPR
CSB-EP379234BPR
CSB-BP379234BPR
CSB-MP379234BPR
CSB-EP379234BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Phosphate acyltransferase (plsX) CSB-YP379235BPR
CSB-EP379235BPR
CSB-BP379235BPR
CSB-MP379235BPR
CSB-EP379235BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei tRNA (guanine-N (1)-)-methyltransferase CSB-YP379236BPR
CSB-EP379236BPR
CSB-BP379236BPR
CSB-MP379236BPR
CSB-EP379236BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei D-amino acid dehydrogenase small subunit (dadA) CSB-YP379237BPR
CSB-EP379237BPR
CSB-BP379237BPR
CSB-MP379237BPR
CSB-EP379237BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei 50S ribosomal protein L33 (rpmG) CSB-YP379238BPR
CSB-EP379238BPR
CSB-BP379238BPR
CSB-MP379238BPR
CSB-EP379238BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB CSB-YP379239BPR
CSB-EP379239BPR
CSB-BP379239BPR
CSB-MP379239BPR
CSB-EP379239BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Probable ubiquinone biosynthesis protein UbiB (ubiB), partial CSB-YP379240BPR1
CSB-EP379240BPR1
CSB-BP379240BPR1
CSB-MP379240BPR1
CSB-EP379240BPR1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Glutamyl-tRNA (Gln) amidotransferase subunit A CSB-YP379241BPR
CSB-EP379241BPR
CSB-BP379241BPR
CSB-MP379241BPR
CSB-EP379241BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Protease HtpX homolog (htpX), partial CSB-YP379242BPR1
CSB-EP379242BPR1
CSB-BP379242BPR1
CSB-MP379242BPR1
CSB-EP379242BPR1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Ribonuclease P protein component (rnpA) CSB-YP379243BPR
CSB-EP379243BPR
CSB-BP379243BPR
CSB-MP379243BPR
CSB-EP379243BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Chromosomal replication initiator protein DnaA (dnaA), partial CSB-YP379244BPR
CSB-EP379244BPR
CSB-BP379244BPR
CSB-MP379244BPR
CSB-EP379244BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Diaminopimelate epimerase (dapF) CSB-YP379245BPR
CSB-EP379245BPR
CSB-BP379245BPR
CSB-MP379245BPR
CSB-EP379245BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei S-adenosylmethionine synthase (metK) CSB-YP379246BPR
CSB-EP379246BPR
CSB-BP379246BPR
CSB-MP379246BPR
CSB-EP379246BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei ATP-dependent dethiobiotin synthetase BioD (bioD) CSB-YP379247BPR
CSB-EP379247BPR
CSB-BP379247BPR
CSB-MP379247BPR
CSB-EP379247BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei 50S ribosomal protein L17 (rplQ) CSB-YP379248BPR
CSB-EP379248BPR
CSB-BP379248BPR
CSB-MP379248BPR
CSB-EP379248BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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