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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Prochlorococcus marinus NAD (P)H-quinone oxidoreductase subunit I CSB-YP381491PZF
CSB-EP381491PZF
CSB-BP381491PZF
CSB-MP381491PZF
CSB-EP381491PZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus tRNA modification GTPase MnmE (mnmE) CSB-YP381492PZF
CSB-EP381492PZF
CSB-BP381492PZF
CSB-MP381492PZF
CSB-EP381492PZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus 50S ribosomal protein L10 (rplJ) CSB-YP381493PZF
CSB-EP381493PZF
CSB-BP381493PZF
CSB-MP381493PZF
CSB-EP381493PZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Biosynthetic arginine decarboxylase (speA), partial CSB-YP381494PZF
CSB-EP381494PZF
CSB-BP381494PZF
CSB-MP381494PZF
CSB-EP381494PZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus DNA ligase (ligA), partial CSB-YP381495PZF
CSB-EP381495PZF
CSB-BP381495PZF
CSB-MP381495PZF
CSB-EP381495PZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus 7-cyano-7-deazaguanine synthase (queC) CSB-YP381496PZF
CSB-EP381496PZF
CSB-BP381496PZF
CSB-MP381496PZF
CSB-EP381496PZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Urease subunit beta (ureB) CSB-YP381497PZF
CSB-EP381497PZF
CSB-BP381497PZF
CSB-MP381497PZF
CSB-EP381497PZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Serologically defined colon cancer antigen 3 homolog (sdccag3) CSB-YP381498DIL
CSB-EP381498DIL
CSB-BP381498DIL
CSB-MP381498DIL
CSB-EP381498DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Calcium-binding mitochondrial carrier protein SCaMC-2-B (slc25a25b), partial CSB-YP381499DIL1
CSB-EP381499DIL1
CSB-BP381499DIL1
CSB-MP381499DIL1
CSB-EP381499DIL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorokybus atmophyticus Peptidoglycan synthase ftsI homolog (ftsI), partial CSB-YP381500CWP1
CSB-EP381500CWP1
CSB-BP381500CWP1
CSB-MP381500CWP1
CSB-EP381500CWP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorokybus atmophyticus 50S ribosomal protein L5, chloroplastic (rpl5) CSB-YP381501CWP
CSB-EP381501CWP
CSB-BP381501CWP
CSB-MP381501CWP
CSB-EP381501CWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Crotalus viridis viridis Zinc metalloproteinase-disintegrin viristiarin CSB-YP381502DYG
CSB-EP381502DYG
CSB-BP381502DYG
CSB-MP381502DYG
CSB-EP381502DYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein SEY1 homolog 1 (TVAG_273580), partial CSB-YP381503TPY
CSB-EP381503TPY
CSB-BP381503TPY
CSB-MP381503TPY
CSB-EP381503TPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Serpin A3-4 (SERPINA3-4) CSB-YP381504BO
CSB-EP381504BO
CSB-BP381504BO
CSB-MP381504BO
CSB-EP381504BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Serpin A3-6 (SERPINA3-6) CSB-YP381505BO
CSB-EP381505BO
CSB-BP381505BO
CSB-MP381505BO
CSB-EP381505BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vitis vinifera Stilbene synthase 6 (STS) CSB-YP381506VFQ
CSB-EP381506VFQ
CSB-BP381506VFQ
CSB-MP381506VFQ
CSB-EP381506VFQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Sperm motility kinase Tcr mutant form (Smoktcr) CSB-YP381507MO
CSB-EP381507MO
CSB-BP381507MO
CSB-MP381507MO
CSB-EP381507MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant NAD (+)--arginine ADP-ribosyltransferase Chelt, partial CSB-YP381508VEW
CSB-EP381508VEW
CSB-BP381508VEW
CSB-MP381508VEW
CSB-EP381508VEW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ipomoea nil CASP-like protein IN26 (IN26), partial CSB-YP381509INC1
CSB-EP381509INC1
CSB-BP381509INC1
CSB-MP381509INC1
CSB-EP381509INC1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Protein YABBY 7 (YAB7) CSB-YP381510OFG
CSB-EP381510OFG
CSB-BP381510OFG
CSB-MP381510OFG
CSB-EP381510OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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