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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Methanoculleus marisnigri Translation initiation factor 5A (eIF5A) CSB-YP387397MNU
CSB-EP387397MNU
CSB-BP387397MNU
CSB-MP387397MNU
CSB-EP387397MNU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanoculleus marisnigri Carbamoyl-phosphate synthase large chain (carB), partial CSB-YP387398MNU
CSB-EP387398MNU
CSB-BP387398MNU
CSB-MP387398MNU
CSB-EP387398MNU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanoculleus marisnigri UPF0290 protein Memar_1002 (Memar_1002), partial CSB-YP387399MNU1
CSB-EP387399MNU1
CSB-BP387399MNU1
CSB-MP387399MNU1
CSB-EP387399MNU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanoculleus marisnigri Proteasome subunit beta (psmB) CSB-YP387400MNU
CSB-EP387400MNU
CSB-BP387400MNU
CSB-MP387400MNU
CSB-EP387400MNU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanoculleus marisnigri UPF0145 protein Memar_1285 (Memar_1285) CSB-YP387401MNU
CSB-EP387401MNU
CSB-BP387401MNU
CSB-MP387401MNU
CSB-EP387401MNU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanoculleus marisnigri Probable 3-phosphoshikimate 1-carboxyvinyltransferase (aroA) CSB-YP387402MNU
CSB-EP387402MNU
CSB-BP387402MNU
CSB-MP387402MNU
CSB-EP387402MNU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanoculleus marisnigri 3-dehydroquinate synthase (Memar_1301) CSB-YP387403MNU
CSB-EP387403MNU
CSB-BP387403MNU
CSB-MP387403MNU
CSB-EP387403MNU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanoculleus marisnigri Dihydrodipicolinate synthase (dapA) CSB-YP387404MNU
CSB-EP387404MNU
CSB-BP387404MNU
CSB-MP387404MNU
CSB-EP387404MNU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanoculleus marisnigri DNA polymerase sliding clamp (pcn) CSB-YP387405MNU
CSB-EP387405MNU
CSB-BP387405MNU
CSB-MP387405MNU
CSB-EP387405MNU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanoculleus marisnigri UPF0280 protein Memar_1519 (Memar_1519) CSB-YP387406MNU
CSB-EP387406MNU
CSB-BP387406MNU
CSB-MP387406MNU
CSB-EP387406MNU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanoculleus marisnigri Probable thymidylate kinase (tmk) CSB-YP387407MNU
CSB-EP387407MNU
CSB-BP387407MNU
CSB-MP387407MNU
CSB-EP387407MNU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanoculleus marisnigri Prefoldin subunit beta (pfdB) CSB-YP387408MNU
CSB-EP387408MNU
CSB-BP387408MNU
CSB-MP387408MNU
CSB-EP387408MNU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanoculleus marisnigri Phosphoribosyl-ATP pyrophosphatase (hisE) CSB-YP387409MNU
CSB-EP387409MNU
CSB-BP387409MNU
CSB-MP387409MNU
CSB-EP387409MNU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanoculleus marisnigri 30S ribosomal protein S13P (rps13p) CSB-YP387410MNU
CSB-EP387410MNU
CSB-BP387410MNU
CSB-MP387410MNU
CSB-EP387410MNU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanoculleus marisnigri Glutamate--tRNA ligase (gltX), partial CSB-YP387411MNU
CSB-EP387411MNU
CSB-BP387411MNU
CSB-MP387411MNU
CSB-EP387411MNU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanoculleus marisnigri L-tyrosine decarboxylase (mfnA) CSB-YP387412MNU
CSB-EP387412MNU
CSB-BP387412MNU
CSB-MP387412MNU
CSB-EP387412MNU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanoculleus marisnigri GMP synthase [glutamine-hydrolyzing] subunit B (guaAB) CSB-YP387413MNU
CSB-EP387413MNU
CSB-BP387413MNU
CSB-MP387413MNU
CSB-EP387413MNU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanoculleus marisnigri Translation initiation factor 6 (eif6) CSB-YP387414MNU
CSB-EP387414MNU
CSB-BP387414MNU
CSB-MP387414MNU
CSB-EP387414MNU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanoculleus marisnigri DNA-binding protein Memar_1972 (Memar_1972) CSB-YP387415MNU
CSB-EP387415MNU
CSB-BP387415MNU
CSB-MP387415MNU
CSB-EP387415MNU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanoculleus marisnigri Protein translation factor SUI1 homolog (Memar_1997) CSB-YP387416MNU
CSB-EP387416MNU
CSB-BP387416MNU
CSB-MP387416MNU
CSB-EP387416MNU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產(chǎn)生大量蛋白質、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產(chǎn)生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們如何確保蛋白質的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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