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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Clostridium thermocellum 30S ribosomal protein S2 (rpsB) CSB-YP387537DUT
CSB-EP387537DUT
CSB-BP387537DUT
CSB-MP387537DUT
CSB-EP387537DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit C CSB-YP387538DUT
CSB-EP387538DUT
CSB-BP387538DUT
CSB-MP387538DUT
CSB-EP387538DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Putative competence-damage inducible protein (cinA) CSB-YP387539DUT
CSB-EP387539DUT
CSB-BP387539DUT
CSB-MP387539DUT
CSB-EP387539DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Ribonuclease Y (rny), partial CSB-YP387540DUT
CSB-EP387540DUT
CSB-BP387540DUT
CSB-MP387540DUT
CSB-EP387540DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Phosphoglucosamine mutase (glmM) CSB-YP387541DUT
CSB-EP387541DUT
CSB-BP387541DUT
CSB-MP387541DUT
CSB-EP387541DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum ATP-dependent Clp protease adapter protein ClpS (clpS) CSB-YP387542DUT
CSB-EP387542DUT
CSB-BP387542DUT
CSB-MP387542DUT
CSB-EP387542DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum 50S ribosomal protein L20 (rplT) CSB-YP387543DUT
CSB-EP387543DUT
CSB-BP387543DUT
CSB-MP387543DUT
CSB-EP387543DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Threonine--tRNA ligase (thrS), partial CSB-YP387544DUT
CSB-EP387544DUT
CSB-BP387544DUT
CSB-MP387544DUT
CSB-EP387544DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Phosphoribosylformylglycinamidine cyclo-ligase (purM) CSB-YP387545DUT
CSB-EP387545DUT
CSB-BP387545DUT
CSB-MP387545DUT
CSB-EP387545DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum UPF0348 protein Cthe_1301 (Cthe_1301) CSB-YP387546DUT
CSB-EP387546DUT
CSB-BP387546DUT
CSB-MP387546DUT
CSB-EP387546DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum D-tyrosyl-tRNA (Tyr) deacylase CSB-YP387547DUT
CSB-EP387547DUT
CSB-BP387547DUT
CSB-MP387547DUT
CSB-EP387547DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Deoxycytidine triphosphate deaminase (dcd) CSB-YP387548DUT
CSB-EP387548DUT
CSB-BP387548DUT
CSB-MP387548DUT
CSB-EP387548DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Urease accessory protein UreE (ureE) CSB-YP387549DUT
CSB-EP387549DUT
CSB-BP387549DUT
CSB-MP387549DUT
CSB-EP387549DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Homoserine O-succinyltransferase (metA) CSB-YP387550DUT
CSB-EP387550DUT
CSB-BP387550DUT
CSB-MP387550DUT
CSB-EP387550DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Arginine--tRNA ligase (argS), partial CSB-YP387551DUT
CSB-EP387551DUT
CSB-BP387551DUT
CSB-MP387551DUT
CSB-EP387551DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Probable transcriptional regulatory protein Cthe_2075 (Cthe_2075) CSB-YP387552DUT
CSB-EP387552DUT
CSB-BP387552DUT
CSB-MP387552DUT
CSB-EP387552DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum UPF0133 protein Cthe_2143 (Cthe_2143) CSB-YP387553DUT
CSB-EP387553DUT
CSB-BP387553DUT
CSB-MP387553DUT
CSB-EP387553DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Flagellar assembly factor FliW (fliW) CSB-YP387554DUT
CSB-EP387554DUT
CSB-BP387554DUT
CSB-MP387554DUT
CSB-EP387554DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum 50S ribosomal protein L9 (rplI) CSB-YP387555DUT
CSB-EP387555DUT
CSB-BP387555DUT
CSB-MP387555DUT
CSB-EP387555DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Ribosomal RNA large subunit methyltransferase H (rlmH) CSB-YP387556DUT
CSB-EP387556DUT
CSB-BP387556DUT
CSB-MP387556DUT
CSB-EP387556DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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