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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bovine Splicing factor 3B subunit 3 (SF3B3), partial CSB-EP021127BO 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Human Bifunctional 3'-5' exonuclease/ATP-dependent helicase WRN (WRN), partial CSB-EP614518HU1a0 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Escherichia coli Transposase for transposon Tn5 (tnpA) (E54K,L372P) CSB-EP669378ENL(M) 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Mouse Apolipoprotein E (Apoe) CSB-EP001936MOc7 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Murine polyomavirus Major capsid protein VP1 CSB-EP326154MKKc7 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Reovirus type 3 Outer capsid protein sigma-3 (S4) CSB-EP365971RCHa0 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Escherichia phage lambda Capsid decoration protein (D) CSB-EP361132FSFe3 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Human Receptor-type tyrosine-protein phosphatase-like N (PTPRN), partial CSB-EP613703HU 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Plasmodium falciparum L-lactate dehydrogenase, partial CSB-EP318413PLO 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Rat Beta-synuclein (Sncb) CSB-EP717413RA 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Epstein-Barr virus Secreted protein BARF1 (BARF1) CSB-EP314065EEZ 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Influenza A virus protein PB1-F2 (PB1) CSB-EP455303IMU 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Human Neuronal acetylcholine receptor subunit alpha-7 (CHRNA7), partial CSB-EP005393HUa0 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Human Egl nine homolog 1 (EGLN1), partial CSB-EP863932HU1 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Human NACHT, LRR and PYD domains-containing protein 3 (NLRP3), partial CSB-EP822275HU2 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Chlamydia trachomatis Major outer membrane porin, serovar D (ompA) CSB-EP671587DSB 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Human Friend leukemia integration 1 transcription factor (FLI1) CSB-EP008714HU 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Human UV excision repair protein RAD23 homolog A (RAD23A) (T131A) CSB-EP019259HU(M) 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Human SH2B adapter protein 1 (SH2B1) (T484A,V541A), partial CSB-EP868300HU1(M) 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Human Nucleosome assembly protein 1-like 4 (NAP1L4) CSB-EP015444HU 1mg(1mg*1 or 500ug*2)/100ug/20ug

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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