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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Corynebacterium glutamicum Glutamyl-tRNA (Gln) amidotransferase subunit A CSB-YP390461CXG
CSB-EP390461CXG
CSB-BP390461CXG
CSB-MP390461CXG
CSB-EP390461CXG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum Glycerol-3-phosphate dehydrogenase [NAD (P)+] CSB-YP390462CXG
CSB-EP390462CXG
CSB-BP390462CXG
CSB-MP390462CXG
CSB-EP390462CXG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum Phosphopantetheine adenylyltransferase (coaD) CSB-YP390463CXG
CSB-EP390463CXG
CSB-BP390463CXG
CSB-MP390463CXG
CSB-EP390463CXG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum N-acetyl-gamma-glutamyl-phosphate reductase (argC) CSB-YP390464CXG
CSB-EP390464CXG
CSB-BP390464CXG
CSB-MP390464CXG
CSB-EP390464CXG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum L-cysteine:1D-myo-inositol 2-amino-2-deoxy-alpha-D-glucopyranoside ligase (mshC) CSB-YP390465CXG
CSB-EP390465CXG
CSB-BP390465CXG
CSB-MP390465CXG
CSB-EP390465CXG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum Putative sporulation transcription regulator WhiA (whiA) CSB-YP390466CXG
CSB-EP390466CXG
CSB-BP390466CXG
CSB-MP390466CXG
CSB-EP390466CXG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum Orotidine 5'-phosphate decarboxylase (pyrF) CSB-YP390467CXG
CSB-EP390467CXG
CSB-BP390467CXG
CSB-MP390467CXG
CSB-EP390467CXG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum Chorismate synthase (aroC) CSB-YP390468CXG
CSB-EP390468CXG
CSB-BP390468CXG
CSB-MP390468CXG
CSB-EP390468CXG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum Aspartate--tRNA ligase (aspS), partial CSB-YP390469CXG
CSB-EP390469CXG
CSB-BP390469CXG
CSB-MP390469CXG
CSB-EP390469CXG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum Transcriptional repressor NrdR (nrdR) CSB-YP390470CXG
CSB-EP390470CXG
CSB-BP390470CXG
CSB-MP390470CXG
CSB-EP390470CXG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum tRNA pseudouridine synthase B (truB) CSB-YP390471CXG
CSB-EP390471CXG
CSB-BP390471CXG
CSB-MP390471CXG
CSB-EP390471CXG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum Ribosome-binding factor A (rbfA) CSB-YP390472CXG
CSB-EP390472CXG
CSB-BP390472CXG
CSB-MP390472CXG
CSB-EP390472CXG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum Proline--tRNA ligase (proS), partial CSB-YP390473CXG
CSB-EP390473CXG
CSB-BP390473CXG
CSB-MP390473CXG
CSB-EP390473CXG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum Probable malate:quinone oxidoreductase (mqo) CSB-YP390474CXG
CSB-EP390474CXG
CSB-BP390474CXG
CSB-MP390474CXG
CSB-EP390474CXG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr) CSB-YP390475CXG
CSB-EP390475CXG
CSB-BP390475CXG
CSB-MP390475CXG
CSB-EP390475CXG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum Uridylate kinase (pyrH) CSB-YP390476CXG
CSB-EP390476CXG
CSB-BP390476CXG
CSB-MP390476CXG
CSB-EP390476CXG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum Thiazole synthase (thiG) CSB-YP390477CXG
CSB-EP390477CXG
CSB-BP390477CXG
CSB-MP390477CXG
CSB-EP390477CXG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum Phosphoribosyl-AMP cyclohydrolase (hisI) CSB-YP390478CXG
CSB-EP390478CXG
CSB-BP390478CXG
CSB-MP390478CXG
CSB-EP390478CXG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum Imidazoleglycerol-phosphate dehydratase (hisB) CSB-YP390479CXG
CSB-EP390479CXG
CSB-BP390479CXG
CSB-MP390479CXG
CSB-EP390479CXG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum Histidinol-phosphate aminotransferase (hisC) CSB-YP390480CXG
CSB-EP390480CXG
CSB-BP390480CXG
CSB-MP390480CXG
CSB-EP390480CXG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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