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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Pelotomaculum thermopropionicum 3-phosphoshikimate 1-carboxyvinyltransferase (aroA) CSB-YP399746PYG
CSB-EP399746PYG
CSB-BP399746PYG
CSB-MP399746PYG
CSB-EP399746PYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelotomaculum thermopropionicum UPF0173 metal-dependent hydrolase PTH_1415 (PTH_1415) CSB-YP399747PYG
CSB-EP399747PYG
CSB-BP399747PYG
CSB-MP399747PYG
CSB-EP399747PYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelotomaculum thermopropionicum DNA mismatch repair protein mutL (mutL), partial CSB-YP399748PYG
CSB-EP399748PYG
CSB-BP399748PYG
CSB-MP399748PYG
CSB-EP399748PYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelotomaculum thermopropionicum Putative competence-damage inducible protein (cinA) CSB-YP399749PYG
CSB-EP399749PYG
CSB-BP399749PYG
CSB-MP399749PYG
CSB-EP399749PYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelotomaculum thermopropionicum Polyribonucleotide nucleotidyltransferase (pnp), partial CSB-YP399750PYG
CSB-EP399750PYG
CSB-BP399750PYG
CSB-MP399750PYG
CSB-EP399750PYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelotomaculum thermopropionicum ATP-dependent protease subunit HslV (hslV) CSB-YP399751PYG
CSB-EP399751PYG
CSB-BP399751PYG
CSB-MP399751PYG
CSB-EP399751PYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelotomaculum thermopropionicum 3-dehydroquinate dehydratase (aroQ) CSB-YP399752PYG
CSB-EP399752PYG
CSB-BP399752PYG
CSB-MP399752PYG
CSB-EP399752PYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelotomaculum thermopropionicum tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP399753PYG
CSB-EP399753PYG
CSB-BP399753PYG
CSB-MP399753PYG
CSB-EP399753PYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelotomaculum thermopropionicum Crossover junction endodeoxyribonuclease RuvC (ruvC) CSB-YP399754PYG
CSB-EP399754PYG
CSB-BP399754PYG
CSB-MP399754PYG
CSB-EP399754PYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelotomaculum thermopropionicum Ribosomal protein L11 methyltransferase (prmA) CSB-YP399755PYG
CSB-EP399755PYG
CSB-BP399755PYG
CSB-MP399755PYG
CSB-EP399755PYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelotomaculum thermopropionicum Trigger factor (tig) CSB-YP399756PYG
CSB-EP399756PYG
CSB-BP399756PYG
CSB-MP399756PYG
CSB-EP399756PYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelotomaculum thermopropionicum Thiamine-phosphate synthase (thiE) CSB-YP399757PYG
CSB-EP399757PYG
CSB-BP399757PYG
CSB-MP399757PYG
CSB-EP399757PYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelotomaculum thermopropionicum Acetaldehyde dehydrogenase (mhpF) CSB-YP399758PYG
CSB-EP399758PYG
CSB-BP399758PYG
CSB-MP399758PYG
CSB-EP399758PYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelotomaculum thermopropionicum 30S ribosomal protein S4 (rpsD) CSB-YP399759PYG
CSB-EP399759PYG
CSB-BP399759PYG
CSB-MP399759PYG
CSB-EP399759PYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelotomaculum thermopropionicum 50S ribosomal protein L29 (rpmC) CSB-YP399760PYG
CSB-EP399760PYG
CSB-BP399760PYG
CSB-MP399760PYG
CSB-EP399760PYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelotomaculum thermopropionicum 30S ribosomal protein S5 (rpsE) CSB-YP399761PYG
CSB-EP399761PYG
CSB-BP399761PYG
CSB-MP399761PYG
CSB-EP399761PYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelotomaculum thermopropionicum 30S ribosomal protein S10 (rpsJ) CSB-YP399762PYG
CSB-EP399762PYG
CSB-BP399762PYG
CSB-MP399762PYG
CSB-EP399762PYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelotomaculum thermopropionicum 50S ribosomal protein L11 (rplK) CSB-YP399763PYG
CSB-EP399763PYG
CSB-BP399763PYG
CSB-MP399763PYG
CSB-EP399763PYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelotomaculum thermopropionicum Type III pantothenate kinase (coaX) CSB-YP399764PYG
CSB-EP399764PYG
CSB-BP399764PYG
CSB-MP399764PYG
CSB-EP399764PYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelotomaculum thermopropionicum Ribosomal RNA small subunit methyltransferase A (rsmA) CSB-YP399765PYG
CSB-EP399765PYG
CSB-BP399765PYG
CSB-MP399765PYG
CSB-EP399765PYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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