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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Cycas taitungensis 50S ribosomal protein L36, chloroplastic (rpl36) CSB-YP405772CXU
CSB-EP405772CXU
CSB-BP405772CXU
CSB-MP405772CXU
CSB-EP405772CXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cycas taitungensis 30S ribosomal protein S7, chloroplastic (rps7-A) CSB-YP405773CXU
CSB-EP405773CXU
CSB-BP405773CXU
CSB-MP405773CXU
CSB-EP405773CXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cycas taitungensis Photosystem I iron-sulfur center (psaC) CSB-YP405774CXU
CSB-EP405774CXU
CSB-BP405774CXU
CSB-MP405774CXU
CSB-EP405774CXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse IQ and AAA domain-containing protein 1-like (Iqca1p1), partial CSB-YP405775MO
CSB-EP405775MO
CSB-BP405775MO
CSB-MP405775MO
CSB-EP405775MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Serine protease 40 (Prss40) CSB-YP405776MO
CSB-EP405776MO
CSB-BP405776MO
CSB-MP405776MO
CSB-EP405776MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Cancer susceptibility candidate protein 1 homolog (casc1), partial CSB-YP405777DIL
CSB-EP405777DIL
CSB-BP405777DIL
CSB-MP405777DIL
CSB-EP405777DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides vulgatus UDP-3-O-acylglucosamine N-acyltransferase (lpxD) CSB-YP405778BOP
CSB-EP405778BOP
CSB-BP405778BOP
CSB-MP405778BOP
CSB-EP405778BOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides vulgatus L-rhamnose mutarotase (rhaM) CSB-YP405779BOP
CSB-EP405779BOP
CSB-BP405779BOP
CSB-MP405779BOP
CSB-EP405779BOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides vulgatus 3-oxoacyl-[acyl-carrier-protein] synthase 3 (fabH) CSB-YP405780BOP
CSB-EP405780BOP
CSB-BP405780BOP
CSB-MP405780BOP
CSB-EP405780BOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides vulgatus Elongation factor P (efp) CSB-YP405781BOP
CSB-EP405781BOP
CSB-BP405781BOP
CSB-MP405781BOP
CSB-EP405781BOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides vulgatus 30S ribosomal protein S4 (rpsD) CSB-YP405782BOP
CSB-EP405782BOP
CSB-BP405782BOP
CSB-MP405782BOP
CSB-EP405782BOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides vulgatus 50S ribosomal protein L6 (rplF) CSB-YP405783BOP
CSB-EP405783BOP
CSB-BP405783BOP
CSB-MP405783BOP
CSB-EP405783BOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides vulgatus 30S ribosomal protein S10 (rpsJ) CSB-YP405784BOP
CSB-EP405784BOP
CSB-BP405784BOP
CSB-MP405784BOP
CSB-EP405784BOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides vulgatus 50S ribosomal protein L1 (rplA) CSB-YP405785BOP
CSB-EP405785BOP
CSB-BP405785BOP
CSB-MP405785BOP
CSB-EP405785BOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides vulgatus Methionine--tRNA ligase (metG), partial CSB-YP405786BOP
CSB-EP405786BOP
CSB-BP405786BOP
CSB-MP405786BOP
CSB-EP405786BOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides vulgatus tRNA pseudouridine synthase B (truB) CSB-YP405787BOP
CSB-EP405787BOP
CSB-BP405787BOP
CSB-MP405787BOP
CSB-EP405787BOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides vulgatus Putative membrane protein insertion efficiency factor (BVU_1189) CSB-YP405788BOP
CSB-EP405788BOP
CSB-BP405788BOP
CSB-MP405788BOP
CSB-EP405788BOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides vulgatus Acetylglutamate kinase (argB) CSB-YP405789BOP
CSB-EP405789BOP
CSB-BP405789BOP
CSB-MP405789BOP
CSB-EP405789BOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides vulgatus UDP-N-acetylmuramate--L-alanine ligase (murC) CSB-YP405790BOP
CSB-EP405790BOP
CSB-BP405790BOP
CSB-MP405790BOP
CSB-EP405790BOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides vulgatus 50S ribosomal protein L31 type B (rpmE2) CSB-YP405791BOP
CSB-EP405791BOP
CSB-BP405791BOP
CSB-MP405791BOP
CSB-EP405791BOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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