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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Thermosipho melanesiensis Proline--tRNA ligase (proS), partial CSB-YP406990TKD
CSB-EP406990TKD
CSB-BP406990TKD
CSB-MP406990TKD
CSB-EP406990TKD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosipho melanesiensis Ribosomal RNA large subunit methyltransferase H (rlmH) CSB-YP406991TKD
CSB-EP406991TKD
CSB-BP406991TKD
CSB-MP406991TKD
CSB-EP406991TKD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosipho melanesiensis ATP-dependent protease subunit HslV (hslV) CSB-YP406992TKD
CSB-EP406992TKD
CSB-BP406992TKD
CSB-MP406992TKD
CSB-EP406992TKD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosipho melanesiensis Aspartate 1-decarboxylase (panD) CSB-YP406993TKD
CSB-EP406993TKD
CSB-BP406993TKD
CSB-MP406993TKD
CSB-EP406993TKD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosipho melanesiensis 50S ribosomal protein L32 (rpmF) CSB-YP406994TKD
CSB-EP406994TKD
CSB-BP406994TKD
CSB-MP406994TKD
CSB-EP406994TKD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosipho melanesiensis Phosphopantetheine adenylyltransferase (coaD) CSB-YP406995TKD
CSB-EP406995TKD
CSB-BP406995TKD
CSB-MP406995TKD
CSB-EP406995TKD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosipho melanesiensis 30S ribosomal protein S18 (rpsR) CSB-YP406996TKD
CSB-EP406996TKD
CSB-BP406996TKD
CSB-MP406996TKD
CSB-EP406996TKD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosipho melanesiensis Glutamate-1-semialdehyde 2,1-aminomutase (hemL) CSB-YP406997TKD
CSB-EP406997TKD
CSB-BP406997TKD
CSB-MP406997TKD
CSB-EP406997TKD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosipho melanesiensis DNA mismatch repair protein mutL (mutL), partial CSB-YP406998TKD
CSB-EP406998TKD
CSB-BP406998TKD
CSB-MP406998TKD
CSB-EP406998TKD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosipho melanesiensis Sugar fermentation stimulation protein homolog (sfsA) CSB-YP406999TKD
CSB-EP406999TKD
CSB-BP406999TKD
CSB-MP406999TKD
CSB-EP406999TKD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosipho melanesiensis 30S ribosomal protein S7 (rpsG) CSB-YP407000TKD
CSB-EP407000TKD
CSB-BP407000TKD
CSB-MP407000TKD
CSB-EP407000TKD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosipho melanesiensis 50S ribosomal protein L29 (rpmC) CSB-YP407001TKD
CSB-EP407001TKD
CSB-BP407001TKD
CSB-MP407001TKD
CSB-EP407001TKD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosipho melanesiensis Translation initiation factor IF-1 (infA) CSB-YP407002TKD
CSB-EP407002TKD
CSB-BP407002TKD
CSB-MP407002TKD
CSB-EP407002TKD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosipho melanesiensis DNA-directed RNA polymerase subunit alpha (rpoA) CSB-YP407003TKD
CSB-EP407003TKD
CSB-BP407003TKD
CSB-MP407003TKD
CSB-EP407003TKD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosipho melanesiensis 50S ribosomal protein L21 (rplU) CSB-YP407004TKD
CSB-EP407004TKD
CSB-BP407004TKD
CSB-MP407004TKD
CSB-EP407004TKD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosipho melanesiensis LexA repressor (lexA) CSB-YP407005TKD
CSB-EP407005TKD
CSB-BP407005TKD
CSB-MP407005TKD
CSB-EP407005TKD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosipho melanesiensis Transcriptional repressor NrdR (nrdR) CSB-YP407006TKD
CSB-EP407006TKD
CSB-BP407006TKD
CSB-MP407006TKD
CSB-EP407006TKD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosipho melanesiensis Ribosome-binding factor A (rbfA) CSB-YP407007TKD
CSB-EP407007TKD
CSB-BP407007TKD
CSB-MP407007TKD
CSB-EP407007TKD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosipho melanesiensis tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP407008TKD
CSB-EP407008TKD
CSB-BP407008TKD
CSB-MP407008TKD
CSB-EP407008TKD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosipho melanesiensis Ribosomal RNA small subunit methyltransferase H (rsmH) CSB-YP407009TKD
CSB-EP407009TKD
CSB-BP407009TKD
CSB-MP407009TKD
CSB-EP407009TKD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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